Dataset Information


Identification of miRNAs and their target genes in developing seeds of soybean by deep sequencing

ABSTRACT: In our study, small RNA library and degradome library were constructed from developing soybean seeds for deep sequencing. We identified 26 new miRNAs in soybean by bioinformatic analysis, and further confirmed their expression by stem-loop RT-PCR. The miRNA star sequences of 38 known miRNAs and 8 new miRNAs were also discovered, providing additional evidence for the existence of miRNAs. Through degradome sequencing, 145 and 25 genes were identified as targets of annotated miRNAs and new miRNAs, respectively. Many identified miRNA targets may perform functions in soybean seed development by GO analysis. Additionally, soybean homolog of Arabidopsis SUPPRESSOR OF GENE SLIENCING 3(AtSGS3) was detected as target of the new identified miRNA Soy_25, suggesting presence of feedback control of miRNA biogenesis sample 1: Examination of small RNA in soybean seed sample 2: identification of miRNA targets in soybean seed

ORGANISM(S): Glycine max  

SUBMITTER: Qing-Xin Song   Jinsong Zhang  Jin-Song Zhang 

PROVIDER: E-GEOD-25260 | ArrayExpress | 2010-11-11



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Identification of miRNAs and their target genes in developing soybean seeds by deep sequencing.

Song Qing-Xin QX   Liu Yun-Feng YF   Hu Xing-Yu XY   Zhang Wan-Ke WK   Ma Biao B   Chen Shou-Yi SY   Zhang Jin-Song JS  

BMC plant biology 20110110

BACKGROUND: MicroRNAs (miRNAs) regulate gene expression by mediating gene silencing at transcriptional and post-transcriptional levels in higher plants. miRNAs and related target genes have been widely studied in model plants such as Arabidopsis and rice; however, the number of identified miRNAs in soybean (Glycine max) is limited, and global identification of the related miRNA targets has not been reported in previous research. RESULTS: In our study, a small RNA library and a degradome library  ...[more]

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