Proteomics

Dataset Information

3

PI3K/mTOR specific phospho-antibody microarray to study the effect of rapamycin to the innate immune respsonse


ABSTRACT: To comprehensively analyze the effects of mTORC1 inhibition on GSK3, we employed the use of a PI3K/mTOR-specific phospho-antibody microarray that analyzed the site-specific phosphorylation of over 130 kinases within the PI3K/mTOR pathway. The phosphorylation levels of different kinases in monocytes were measured when stimulated with LPS in the presence or absence of a kind of mTORC1 inhibitor, rapamycin More than 130 highly specific and characterized phospho-antibodies for the human mTOR signaling pathway were immobilized and replicated six times on glass slides. The same non-phosphorylated target antibodies were included to allow the determination of the relative level of phosphorylatioin

ORGANISM(S): Homo sapiens  

SUBMITTER: Huizhi wang   Ruqiang Liang  huizhi wang  Michael Martin 

PROVIDER: E-GEOD-25520 | ArrayExpress | 2010-11-25

SECONDARY ACCESSION(S): GSE25520PRJNA133777

REPOSITORIES: GEO, ArrayExpress

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