Transcriptomics,Genomics

Dataset Information

0

Polycomb Protein Ezh1 Promotes RNA Polymerase II Elongation


ABSTRACT: Polycomb group (PcG) proteins initiate the formation of repressed chromatin domains and regulate developmental gene expression. A mammalian PcG protein, Enhancer of Zeste homolog 2 (Ezh2), triggers transcriptional repression by catalyzing the addition of methyl groups onto lysine-27 of histone H3 (H3K27me2/3)1. This action facilitates the binding of other PcG proteins to histone H3 and compaction of chromatin. Interestingly, there exists a paralog of Ezh2, termed Ezh1, whose primary function remains unclear. Here, we provide evidence for genome-wide association of Ezh1 with active epigenetic marks, RNA polymerase II (PolII) and mRNA production. Ezh1 depletion reduced global PolII occupancy within gene bodies and resulted in delayed transcriptional activation during differentiation of skeletal muscle cells. Conversely, ectopic expression of wild-type Ezh1 led to premature gene activation and rescued PolII-elongation defects in Ezh1-depleted cells. Collectively, these findings reveal an unanticipated role of a PcG protein in promoting mRNA transcription. Examination of 3 different histone modifications, 3 modified forms of RNA polymerase II, Ezh1, Ezh2 and mRNA levels in a skeletal muscle cells at various developmental stages.

ORGANISM(S): Mus musculus  

SUBMITTER: Vittorio Sartorelli   Kambiz Mousavi  Hossein Zare  A H Wang 

PROVIDER: E-GEOD-25549 | ArrayExpress | 2011-11-23

SECONDARY ACCESSION(S): SRP004638SRP004636GSE25549PRJNA134029

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

Polycomb protein Ezh1 promotes RNA polymerase II elongation.

Mousavi Kambiz K   Zare Hossein H   Wang A Hongjun AH   Sartorelli Vittorio V  

Molecular cell 20111222 2


Polycomb group (PcG) proteins initiate the formation of repressed chromatin domains and regulate developmental gene expression. A mammalian PcG protein, enhancer of zeste homolog 2 (Ezh2), triggers transcriptional repression by catalyzing the addition of methyl groups onto lysine 27 of histone H3 (H3K27me2/3). This action facilitates the binding of other PcG proteins to chromatin for purposes of transcriptional silencing. Interestingly, there exists a paralog of Ezh2, termed Ezh1, whose primary  ...[more]

Similar Datasets

2011-11-23 | GSE25549 | GEO
2011-06-09 | E-GEOD-26616 | ArrayExpress
| GSE95225 | GEO
| GSE59346 | GEO
2011-06-09 | GSE26616 | GEO
2013-04-01 | E-GEOD-44119 | ArrayExpress
2014-10-30 | E-GEOD-62437 | ArrayExpress
2015-08-31 | E-GEOD-70440 | ArrayExpress
| GSE39450 | GEO
2011-12-27 | E-GEOD-32929 | ArrayExpress