Transcriptomics

Dataset Information

49

Comparing Control and Schizophrenic hiPSC-derived Neurons


ABSTRACT: Schizophrenia is a debilitating neurological disorder for which no cure exists. Few defining characteristics of schizophrenic neurons have been identified and the molecular mechanisms responsible for schizophrenia are not well understood, in part due to the lack of patient material for study. Human induced pluripotent stem cells (hiPSCs) offer a new strategy for studying schizophrenia. We have created the first cell-based human model of a complex genetic psychiatric disease by generating hiPSCs from schizophrenic patients and subsequently differentiating these cells to hiPSC-derived neurons in vitro. Schizophrenic hiPSC-derived neurons showed diminished neuronal connectivity in conjunction with decreased neurite number, PSD95-protein levels and glutamate receptor expression. Gene expression profiles of schizophrenic hiPSC-derived neurons identified altered expression of many components of the cAMP and WNT signaling pathways. Key cellular and molecular elements of the schizophrenic phenotype were ameliorated following treatment of schizophrenic hiPSC-derived neurons with the antipsychotic loxapine. 3 independent differentiations (biological replicates) for each of four control and four schizophrenic patients were analyzed.

ORGANISM(S): Homo sapiens  

SUBMITTER: Kristen J Brennand  Fred H Gage   Kristen Brennand    

PROVIDER: E-GEOD-25673 | ArrayExpress| 2012-03-27

SECONDARY ACCESSION(S): GSE25673PRJNA133867

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications


Schizophrenia (SCZD) is a debilitating neurological disorder with a world-wide prevalence of 1%; there is a strong genetic component, with an estimated heritability of 80-85%. Although post-mortem studies have revealed reduced brain volume, cell size, spine density and abnormal neural distribution in the prefrontal cortex and hippocampus of SCZD brain tissue and neuropharmacological studies have implicated dopaminergic, glutamatergic and GABAergic activity in SCZD, the cell types affected in SCZ  ...[more]

Similar Datasets

2011-05-01 | GSE25673 | GEO
2014-04-09 | E-GEOD-40102 | ArrayExpress
2014-10-10 | E-GEOD-62105 | ArrayExpress
| GSE63734 | GEO
2016-03-19 | E-GEOD-63738 | ArrayExpress
| PRJNA133867 | ENA
2009-08-13 | GSE17612 | GEO
2012-03-27 | E-GEOD-17612 | ArrayExpress
2011-12-31 | GSE21935 | GEO
2012-06-26 | E-GEOD-21935 | ArrayExpress