Transcriptomics

Dataset Information

5

Expression Profiling of Erythroid Progenitors After MYB shRNA Knockdown


ABSTRACT: MYB plays a critical role as a regulator of erythropoieisis. We have shown that MYB silences epsilon and gamma-globin expression in erythroid progenitors. We here examine erythroid cells at the basophilic erythroblast stage of differentiation with MYB shRNA or control lentiviral transduction prior to differentiation. We have cultured CD34 cells and transduced the cells with lentiviruses harboring shRNAs targeting MYB or controls and then allowed the cells to differentiate down the erythroid lineage. At the basophilic erythroblast stage of differentiation, the cells were harvested and total RNA was extracted. This was used to hybridize to Affymetrix expression arrays using the HG-U133 Plus 2.0 platform to ascertain expression differences that occur with the knockdown of MYB.

ORGANISM(S): Homo sapiens  

SUBMITTER: Vijay G Sankaran   Vijay G. Sankaran 

PROVIDER: E-GEOD-25678 | ArrayExpress | 2011-01-03

SECONDARY ACCESSION(S): GSE25678PRJNA135825

REPOSITORIES: GEO, ArrayExpress

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Publications

MicroRNA-15a and -16-1 act via MYB to elevate fetal hemoglobin expression in human trisomy 13.

Sankaran Vijay G VG   Menne Tobias F TF   Šćepanović Danilo D   Vergilio Jo-Anne JA   Ji Peng P   Kim Jinkuk J   Thiru Prathapan P   Orkin Stuart H SH   Lander Eric S ES   Lodish Harvey F HF  

Proceedings of the National Academy of Sciences of the United States of America 20110104 4


Many human aneuploidy syndromes have unique phenotypic consequences, but in most instances it is unclear whether these phenotypes are attributable to alterations in the dosage of specific genes. In human trisomy 13, there is delayed switching and persistence of fetal hemoglobin (HbF) and elevation of embryonic hemoglobin in newborns. Using partial trisomy cases, we mapped this trait to chromosomal band 13q14; by examining the genes in this region, two microRNAs, miR-15a and -16-1, appear as top  ...[more]

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