Transcriptomics

Dataset Information

241

A Genome-Wide Characterization of New and Drought Stress Responsive MicroRNAs in Populus euphratica


ABSTRACT: We present an efficient method to genome-wide discover new and drought stress responsive miRNAs in P. euphratica. High throughput sequencing of P. euphratica leaves found 197 conserved miRNAs between P. euphratica and Populus trichocarpa. Meanwhile, 189 new miRNAs which belonged to 120 families were identified, a large increasing to the number of P. euphratica miRNAs. Target prediction and degradome sequencing verification of 22 new and 21 conserved miRNA targets showed these targets were involved in multiple biological processes, including transcription regulation and response to stimulus. Furthermore, comparison of high-throughput sequencing with miRNA microarray profiling data indicated that 104 miRNA sequences were up-regulated, while 27 were down-regulated under drought stress. This preliminary characterization based on our findings provided a framework for future analysis of miRNA genes and their roles in key traits of poplar as stress resistance plant breeding and environment protection usage. Examination of sRNA expression in 2 poplar leaf samples in drought and normal growth conditions.

ORGANISM(S): Populus euphratica  

SUBMITTER: Bosheng Li   Xinli Xia  bosheng Li 

PROVIDER: E-GEOD-25747 | ArrayExpress | 2011-04-25

SECONDARY ACCESSION(S): SRP004786GSE25747PRJNA134103

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Genome-wide characterization of new and drought stress responsive microRNAs in Populus euphratica.

Li Bosheng B   Qin Yurong Y   Duan Hui H   Yin Weilun W   Xia Xinli X  

Journal of experimental botany 20110421 11


MicroRNAs (miRNAs) are small, non-coding RNAs that play essential roles in plant growth, development, and stress response. Populus euphratica is a typical abiotic stress-resistant woody species. This study presents an efficient method for genome-wide discovery of new drought stress responsive miRNAs in P. euphratica. High-throughput sequencing of P. euphratica leaves found 197 conserved miRNAs between P. euphratica and Populus trichocarpa. Meanwhile, 58 new miRNAs belonging to 38 families were i  ...[more]

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