Transcriptomics

Dataset Information

30

Gene expression profiling of uterine stromal cells isolated from Hand2 floxed and Hand2 ablated mice on day 4 of pregnancy


ABSTRACT: Our previous study revealed that the basic helix-loop-helix transcription factor Hand2 is a downstream target of progesterone signaling in mouse uterine stroma at the time of implantation. Further, conditional deletion of Hand2 in mouse uterus leads to implantation failure due to impaired uterine epithelial receptivity. To identify the downstream targets of Hand2 in the uterus, we performed gene expression profling of uterine stromal cells isolated from Hand2-null mice and the corresponding controls on day4 of pregnancy (the time of implantation). The microarray results revealed elevated expression of mRNAs corresponding to several members of the fibroblast growth factor family in uterine stroma of Hand2-ablated mice. These factors act as paracrine mediators of mitogenic effects of estrogen on the epithleium. Thus, Hand2 is a critical regulator of the uteirne stromal-epithelial communication that directs proper steroid regulation conducive for establshment of pregnancy. We performed conditional ablation of Hand2 in the mouse uterus using the PRcre mouse model. As Hand2 expression is restricted to stromal comaprtment, we isolated uterine stromal cells from day4 pregnant mice (n=5 for each genotype), purified total RNA from these cells, pooled these samples and then hybridized to high density affymetrix microarrays. Control vs. KO.

ORGANISM(S): Mus musculus  

SUBMITTER: Quanxi Li  Indrani C Bagchi   Milan K Bagchi    

PROVIDER: E-GEOD-25881 | ArrayExpress| 2012-03-27

SECONDARY ACCESSION(S): GSE25881PRJNA135811

REPOSITORIES: GEO, ArrayExpress

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Publications

The antiproliferative action of progesterone in uterine epithelium is mediated by Hand2.

Li Quanxi Q   Kannan Athilakshmi A   DeMayo Francesco J FJ   Lydon John P JP   Cooke Paul S PS   Yamagishi Hiroyuki H   Srivastava Deepak D   Bagchi Milan K MK   Bagchi Indrani C IC  

Science (New York, N.Y.) 20110201 6019


During pregnancy, progesterone inhibits the growth-promoting actions of estrogen in the uterus. However, the mechanism for this is not clear. The attenuation of estrogen-mediated proliferation of the uterine epithelium by progesterone is a prerequisite for successful implantation. Our study reveals that progesterone-induced expression of the basic helix-loop-helix transcription factor Hand2 in the uterine stroma suppresses the production of several fibroblast growth factors (FGFs) that act as pa  ...[more]

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