Transcriptomics

Dataset Information

100

Expression changes after loss of Dot1l in murine MLL-AF9 leukemia cells


ABSTRACT: MLL-fusions may induce leukemogenic gene expression programs by recruiting the histone H3K79 methyltransferase to MLL-target promoters. We evaluated gene expression changes after cre-mediated loss of Dot1l in leukemia cells obtained from mice injected with MLL-9 transformed lineage negative bone marrow cells. MLL-AF9 murine leukemia cells carrying two conditional Dot1l alleles were retrovirally transduced with Cre or empty control vector, and gene expression changes were monitored on day 3, 5, and 7 after transduction.

ORGANISM(S): Mus musculus  

SUBMITTER: Kathrin Bernt   Scott Armstrong  Amit Sinha 

PROVIDER: E-GEOD-25911 | ArrayExpress | 2011-09-12

SECONDARY ACCESSION(S): GSE25911PRJNA135857

REPOSITORIES: GEO, ArrayExpress

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Publications


The histone 3 lysine 79 (H3K79) methyltransferase Dot1l has been implicated in the development of leukemias bearing translocations of the Mixed Lineage Leukemia (MLL) gene. We identified the MLL-fusion targets in an MLL-AF9 leukemia model, and conducted epigenetic profiling for H3K79me2, H3K4me3, H3K27me3, and H3K36me3 in hematopoietic progenitor and leukemia stem cells (LSCs). We found abnormal profiles only for H3K79me2 on MLL-AF9 fusion target loci in LSCs. Inactivation of Dot1l led to downre  ...[more]

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