Transcriptomics

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RL-SAGE analysis of Zea mays leaf transcriptome


ABSTRACT: Using the RL-SAGE method (Gowda et al. 2004), a maize leaf longSAGE library (cv. inbred line B73) was constructed. Leaf tissues were harvested from 4-week old B73 plants for RNA isolation. The conditions in the growth chamber were 12 h light (500 µmol photons m-2 sec-1), 20oC at night, 26oC in the day and 85% relative humidity. A total of 44,870 unique tags (17 bases +CATG) were identified from 232,948 individual tags in the maize leaf library. This GEO Series was created by the GEO staff as part of a cleanup effort to ensure that all GEO Samples are included within a Series entry.

ORGANISM(S): Zea mays  

SUBMITTER: Guo-Liang Wang  

PROVIDER: E-GEOD-25994 | ArrayExpress | 2010-12-10

SECONDARY ACCESSION(S): GSE25994PRJNA135425

REPOSITORIES: GEO, ArrayExpress

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Publications

Robust-LongSAGE (RL-SAGE): a substantially improved LongSAGE method for gene discovery and transcriptome analysis.

Gowda Malali M   Jantasuriyarat Chatchawan C   Dean Ralph A RA   Wang Guo-Liang GL  

Plant physiology 20040301 3


Serial analysis of gene expression (SAGE) is a widely used technique for large-scale transcriptome analysis in mammalian systems. Recently, a modified version called LongSAGE (S. Saha, A.B. Sparks, C. Rago, V. Akmaev, C.J. Wang, B. Vogelstein, K.W. Kinzler [2002] Nat Biotechnol 20: 508-512) was reported by increasing tag length up to 21 bp. Although the procedures for these two methods are similar, a detailed protocol for LongSAGE library construction has not been reported yet, and several techn  ...[more]

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