Transcriptomics

Dataset Information

2

Transcriptional profiling of ex vivo isolated inflammed mouse lymphatic endothelial cells


ABSTRACT: Chemokines and adhesion molecules upregulated in lymphatic endothelial cells (LECs) during tissue inflammation are believed to enhance dendritic cell (DC) migration to draining lymph nodes (dLNs), but the in vivo control of this process is not well understood. By performing transcriptional profiling of LECs isolated from murine skin, we found that inflammation induced by a contact hypersensitivity (CHS) response upregulated the adhesion molecules ICAM-1 and VCAM-1 and inflammatory chemokines in LECs. Furthermore, lymphatic lineage markers like Prox-1, VEGFR3 and LYVE-1 were significantly downregulated during CHS. By contrast, skin inflammation induced by Complete Freund’s adjuvant (CFA) induced a different pattern of chemokine and lymphatic marker gene expression and almost no ICAM-1 up-regulation in LECs. In FITC painting experiments, DC migration to dLNs was more strongly increased in CFA- as compared to CHS-induced inflammation. Interestingly, DC migration did not correlate with the induction of CCL21 and ICAM-1 in LECs. However, the requirement for CCR7 signaling became further pronounced during inflammation, whereas CCR7-independent signals only had a minor role in enhancing DC migration. Collectively, these findings indicate that inflammation-induced DC migration is stimulus-dependent and only moderately enhanced by LEC-induced genes other than CCL21. Mouse ear skin single-cell suspensions were prepared by a fast protocol that minimizes the RNA degradation. Fluorescence-activated cell sorting (FACS) was used to sort lymphatic endothelial cells (LEC) from CHS inflammed and control skin. 4 pairs (each with one control and one CHS inflammed sample, sorted and extracted on the same day) of LECs were chosen based on the quality of extracted and amplified material. This gave 8 samples to analyze (4 biological replicates in each condition). Each sample was sorted from 3 mice.

ORGANISM(S): Mus musculus  

SUBMITTER: benjamin vigl   Benjamin Vigl  Cornelia Halin 

PROVIDER: E-GEOD-26229 | ArrayExpress | 2011-05-19

SECONDARY ACCESSION(S): GSE26229PRJNA134995

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Tissue inflammation modulates gene expression of lymphatic endothelial cells and dendritic cell migration in a stimulus-dependent manner.

Vigl Benjamin B   Aebischer David D   Nitschké Maximilian M   Iolyeva Maria M   Röthlin Tamara T   Antsiferova Olga O   Halin Cornelia C  

Blood 20110519 1


Chemokines and adhesion molecules up-regulated in lymphatic endothelial cells (LECs) during tissue inflammation are thought to enhance dendritic cell (DC) migration to draining lymph nodes, but the in vivo control of this process is not well understood. We performed a transcriptional profiling analysis of LECs isolated from murine skin and found that inflammation induced by a contact hypersensitivity (CHS) response up-regulated the adhesion molecules ICAM-1 and VCAM-1 and inflammatory chemokines  ...[more]

Similar Datasets

2011-05-20 | GSE26229 | GEO
2006-12-05 | GSE6257 | GEO
2011-04-01 | GSE28234 | GEO
2011-03-31 | E-GEOD-28234 | ArrayExpress
2008-11-07 | E-GEOD-6257 | ArrayExpress
2010-02-03 | E-MEXP-2452 | ArrayExpress
2009-04-23 | E-GEOD-7694 | ArrayExpress
2014-10-20 | E-GEOD-62510 | ArrayExpress
| GSE62748 | GEO
2013-11-04 | E-GEOD-49358 | ArrayExpress