Dataset Information


Transcription profiling by array of Arabidopsis Col-0 and Ws ecotypes and QK mutants after heat-shock treatment

ABSTRACT: In order to study the importance of HSFA1 in thermotolerance in Arabidopsis, we generated the HSFA1a, b, d and e quadruple mutant (QK). QK is very sensitive to heat. Therefore, we used microarray to study how many genes regulated by HSFA1 after heat shock. Seven-day-old seedlings of Col-0, Ws and QK grown at 22oC on 0.5x MS plates containing 1% sucrose were incubated for 1 h at 37 °C. Subsequently, samples were collected for RNA extraction. The experiment was repeated, and two biological replicates were processed for analysis.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Yee-yung Charng   Hsiang-chin Liu 

PROVIDER: E-GEOD-26266 | ArrayExpress | 2010-12-23



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The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis.

Liu Hsiang-Chin HC   Liao Hsiu-Ting HT   Charng Yee-Yung YY  

Plant, cell & environment 20110225 5

In Arabidopsis, there are four homologs of class A1 heat shock factor (HSFA1) genes, which likely encode the master regulators of heat shock response (HSR). However, previous studies with double knockout (KO) mutants were unable to confirm this point probably due to functional redundancy. Here, we generated a quadruple KO (QK) and four triple KO mutants to dissect their functions. Our data show that members of the HSFA1 group not only play a pivotal role in HSR but also are involved in growth an  ...[more]

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