Transcriptomics

Dataset Information

6

Expression data for Mobilized Peripheral Blood CD34+ cells with Wnt or BMP stimulation


ABSTRACT: Analysis of mobilized peripheral blood CD34+ cells from a healthy volunteer under erythroid differentiation conditions with and without stimulation to the BMP or Wnt signaling pathways. For erythroid differentiation, expanded CD34+ cells were placed in Stemspan SFEM medium supplemented with 2% pen/strep, 20ng/ml SCF, 1U/ml Epo, 5ng/ml IL3, 2uM dexamethasone, and 1uM beta-estradiol. Arrays were performed 2 hours after addition of cytokines. For signaling pathway stimulation, cells were exposed to 0.5uM BIO (a GSK3 inhibitor) for Wnt pathway activation, 25ng/ml rhBMP4 for BMP pathway activation, or vehicle control for 2 hours. Three biological replicates were performed per treatment group. We used microarrays to detail the global program of gene expression changes after Wnt or BMP pathway stimulation in human CD34+ hematopoietic progenitors under erythroid differentiation conditions. To investigate the changes to gene expression in human CD34+ hematopoietic progenitors following stimulation of the Wnt or BMP pathways during the early stages of erythroid differentiation. Three biological replicates were performed per treatment group.

ORGANISM(S): Homo sapiens  

SUBMITTER: Eirini Trompouki   Teresa V Bowman  Robert F Paulson  Teresa Venezia Bowman  Anthony DiBiase  Anna K Sessa  Pulin Li  Richard A Young  Ellen M Durand  Lee N Lawton  George Q Daley  Christian Mosimann  Zi P Fan  Leonard I Zon  Dai-Chen Wu  Garrett C Heffner  Jennifer N Cech  Corey S Martin  Jocelyn L Leblanc 

PROVIDER: E-GEOD-26351 | ArrayExpress | 2011-10-06

SECONDARY ACCESSION(S): GSE26351PRJNA136733

REPOSITORIES: GEO, ArrayExpress

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