Dataset Information


Study of mating type genes and their roles in mating interactions of the basidiomycete Schizophyllum commune

ABSTRACT: Transcriptome changes associated with mating interactions were performed in order to identify signaling components and targets of pheromone response. Two monokaryons (S. commune 12-43 and 4-39), a dikaryon (W22 x 12-43), both semi-compatible mating interactions (Aon: W22 x 4-39; Bon, flat: W21 x 4-39), and different S. commune pheromone receptor recipient strains (Vbar2f, Vbar2t) were analyzed for that purpose. In addition, a S. commune strain showing the thin-phenotype (W22-thin) was investigated to figure out the role of Thn1 in mating. With microarray analysis mRNA expression in different mating interactions of the basidiomycete S. commune was analyzed. Genes which show regulation in the comparison of the two monokaryons 12-43 and 4-39 has been eliminated from other comparisons as strain specific differences. Two biological or technical replicate samples were analyzed for each condition.

ORGANISM(S): Schizophyllum commune  

SUBMITTER: Daniela Freihorst   Erika Kothe  Susann Erdmann 

PROVIDER: E-GEOD-26401 | ArrayExpress | 2012-06-14



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Transcriptome and functional analysis of mating in the basidiomycete Schizophyllum commune.

Erdmann Susann S   Freihorst Daniela D   Raudaskoski Marjatta M   Schmidt-Heck Wolfgang W   Jung Elke-Martina EM   Senftleben Dominik D   Kothe Erika E  

Eukaryotic cell 20111230 5

In this study, we undertook a functional characterization and transcriptome analysis that enabled a comprehensive study of the mating type loci of the mushroom Schizophyllum commune. Induced expression of both the bar2 receptor and the bap2(2) pheromone gene within 6 to 12 h after mates' contact was demonstrated by quantitative real-time PCR. Similar temporal expression patterns were confirmed for the allelic bbr1 receptor and bbp1 pheromone-encoding genes by Northern hybridization. Interestingl  ...[more]

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