Transcriptomics,Multiomics

Dataset Information

2

Replicative senescence of mesenchymal stem cells causes DNA methylation changes which correlate with repressive histone marks


ABSTRACT: Cells in culture undergo replicative senescence. In this study, we analyzed functional, genetic and epigenetic sequels of long‐term culture in human mesenchymal stem cells (MSC). Already within early passages the fibroblastoid colony‐ forming unit (CFU‐f) frequency and the differentiation potential of MSC declined significantly. Relevant chromosomal aberrations were not detected by karyotyping and SNP‐microarrays. Subsequently, we have compared DNA‐methylation profiles with the Infinium HumanMethylation27 Bead Array and the profiles differed markedly in MSC derived from adipose tissue and bone marrow. Notably, all MSC revealed highly consistent senescence‐associated modifications at specific CpG sites. These DNA‐methylation changes correlated with histone marks of previously published data sets, such as trimethylation of H3K9, H3K27 and EZH2 targets. Taken together, culture expansion of MSC has profound functional implications ‐ these are hardly reflected by genomic instability but they are associated with highly reproducible DNA‐methylation changes which correlate with repressive histone marks. Therefore replicative senescence seems to be epigenetically controlled. 8 samples of mesenchymal stem cells (MSC) from human adipose tissue

OTHER RELATED OMICS DATASETS IN: PRJNA136709S-EPMC7075810PRJNA137655S-EPMC7026953

ORGANISM(S): Homo sapiens  

SUBMITTER: Sylvia Joussen   Herdit Schüler  Gudrun Bokermann  Anne Schellenberg  Qiong Lin  Christoph V Suschek  Bernd Denecke  Martin Zenke  Norbert Pallua  Carmen Koch  Wolfgang Wagner 

PROVIDER: E-GEOD-26519 | ArrayExpress | 2011-10-27

SECONDARY ACCESSION(S): GSE26519PRJNA136709

REPOSITORIES: GEO, ArrayExpress

Dataset's files

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E-GEOD-26519.README.txt Txt
E-GEOD-26519.idf.txt Idf
E-GEOD-26519.processed.1.zip Processed
E-GEOD-26519.sdrf.txt Txt
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Publications

Replicative senescence of mesenchymal stem cells causes DNA-methylation changes which correlate with repressive histone marks.

Schellenberg Anne A   Lin Qiong Q   Schüler Herdit H   Koch Carmen M CM   Joussen Sylvia S   Denecke Bernd B   Walenda Gudrun G   Pallua Norbert N   Suschek Christoph V CV   Zenke Martin M   Wagner Wolfga W  

Aging 20110901 9


Cells in culture undergo replicative senescence. In this study, we analyzed functional, genetic and epigenetic sequels of long-term culture in human mesenchymal stem cells (MSC). Already within early passages the fibroblastoid colony-forming unit (CFU-f) frequency and the differentiation potential of MSC declined significantly. Relevant chromosomal aberrations were not detected by karyotyping and SNP-microarrays. Subsequently, we have compared DNA-methylation profiles with the Infinium HumanMeth  ...[more]

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