Dataset Information


Expression data from differentiating Flk1- and Flk1+ ES cells expressing Snail during Wnt inhibition

ABSTRACT: ES cells differentiated in the presence of the Wnt inhibitor DKK1 fail to express the transcription factor Snail and undergo EMT or mesoderm differentiation. We generated an ES cell line, A2.snail, that induced Snail expression upon addition of doxycycline addition. Microarrays were used to gain a global picture of Flk1- and Flk1+ cells generated one day after Snail was expressed during Wnt inhibition. A2.snail ES cells, which express Snail upon addition of doxycycline, were differentiated as embryoid bodies in differentiation media and DKK1. Snail-induced cultures uniquely develop a select population of Flk1+ cells. Total RNA was harvested from sorted control (no doxycycline) Flk1- cells and sorted Snail-induced (doxycycline at day 2) Flk1- and Flk1+ cells at day 3 of differentiation.

ORGANISM(S): Mus musculus  

SUBMITTER: Theresa L Murphy   Ellen M Langer  Robert C Lindsley  Kenneth M Murphy  Jennifer Gill  Mi Cai  Jennifer G Gill  Michael Kyba 

PROVIDER: E-GEOD-26524 | ArrayExpress | 2013-10-23



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Snail promotes the cell-autonomous generation of Flk1(+) endothelial cells through the repression of the microRNA-200 family.

Gill Jennifer G JG   Langer Ellen M EM   Lindsley R Coleman RC   Cai Mi M   Murphy Theresa L TL   Murphy Kenneth M KM  

Stem cells and development 20111018 2

Expression of the transcription factor Snail is required for normal vasculogenesis in the developing mouse embryo. In addition, tumors expressing Snail have been associated with a more malignant phenotype, with both increased invasive properties and angiogenesis. Although the relationship between Snail and vasculogenesis has been noted, no mechanistic analysis has been elucidated. Here, we show that in addition to inducing an epithelial mesenchymal transition, Snail promotes the cell-autonomous  ...[more]

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