Transcriptomics

Dataset Information

5

Changes in miR expression profile carotid plaques induced by acutely induced flow disturbances in apoe-/- mice on a high fat diet.


ABSTRACT: Atherosclerosis is causally related to disturbed flow through low and oscillatory shear stress. In order to study the miR expression profile in atherosclerotic plaques induced by disturbed flow, partial ligation of the carotid artery was performed. This procedure results acutely in severly reduced blood flow and in stenotic lesion formation within 6 weeks in apoe-/- mice on a high fat diet. We compared the miR expression profile in partially ligated left carotid arteries with the untreated right carotid artery to identify miRs which are involved in plaque formation through flow disturbances. The left carotid arteries of 6 female apoe-/- mice (6-8 weeks) were partially ligated (i.e. the external and internal carotid artery as well as the occipital artery were occluded; blood flow out of the common carotid artery occurs mainly through the superior thyroid artery). Following partial ligation the animals were fed a high fat diet for 6 weeks. Total RNA was isolated from partially ligated left carotid arteries and untreated right carotid arteries (control). MiRs expression profile of the partially ligated carotid arteries were compared with the control group. Biological replicates: 6 per group. One replicate per array.

ORGANISM(S): Mus musculus  

SUBMITTER: Andreas Schober   Malihe Nazari Jahantigh 

PROVIDER: E-GEOD-26555 | ArrayExpress | 2012-12-26

SECONDARY ACCESSION(S): GSE26555PRJNA142281

REPOSITORIES: GEO, ArrayExpress

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Publications

MicroRNA-155 promotes atherosclerosis by repressing Bcl6 in macrophages.

Nazari-Jahantigh Maliheh M   Wei Yuanyuan Y   Noels Heidi H   Akhtar Shamima S   Zhou Zhe Z   Koenen Rory R RR   Heyll Kathrin K   Gremse Felix F   Kiessling Fabian F   Grommes Jochen J   Weber Christian C   Schober Andreas A  

The Journal of clinical investigation 20121008 11


Macrophages in atherosclerotic plaques drive inflammatory responses, degrade lipoproteins, and phagocytose dead cells. MicroRNAs (miRs) control the differentiation and activity of macrophages by regulating the signaling of key transcription factors. However, the functional role of macrophage-related miRs in the immune response during atherogenesis is unknown. Here, we report that miR-155 is specifically expressed in atherosclerotic plaques and proinflammatory macrophages, where it was induced by  ...[more]

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