Transcriptomics

Dataset Information

298

Transcriptional Regulation of Anabaena 7120 response to Nitrogen Deprivation


ABSTRACT: Here, we report the transcriptome of Anabaena sp. strain 7120, a cyanobacterium that forms specialized nitrogen-fixing cells called heterocysts. Our data suggests that cyanobacteria frequently have more complex transcripts than thought, with large 5' UTRs, numerous antisense transcripts, and multiple transcriptional start sites or processing sites. Four samples of total filament RNA were sequenced with Illumina 40bp reads using directional RNA sequencing (see the Illumina small RNA prep protocol). The samples are 0hr (vegetative cells grown in the presence of ammonia) and 6hr, 12hr, and 21hr cells (after nitrogen step down).

ORGANISM(S): Nostoc sp. PCC 7120  

SUBMITTER: Britt L Flaherty   James W Golden  F Van Nieuwerburgh  Steven R Head 

PROVIDER: E-GEOD-26633 | ArrayExpress | 2011-06-22

SECONDARY ACCESSION(S): SRP005431GSE26633PRJNA136453

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Directional RNA deep sequencing sheds new light on the transcriptional response of Anabaena sp. strain PCC 7120 to combined-nitrogen deprivation.

Flaherty Britt L BL   Van Nieuwerburgh F F   Head Steven R SR   Golden James W JW  

BMC genomics 20110628


Cyanobacteria are potential sources of renewable chemicals and biofuels and serve as model organisms for bacterial photosynthesis, nitrogen fixation, and responses to environmental changes. Anabaena (Nostoc) sp. strain PCC 7120 (hereafter Anabaena) is a multicellular filamentous cyanobacterium that can "fix" atmospheric nitrogen into ammonia when grown in the absence of a source of combined nitrogen. Because the nitrogenase enzyme is oxygen sensitive, Anabaena forms specialized cells called hete  ...[more]

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