Transcriptomics

Dataset Information

5

Isolation of epiblast stem cells from pre-implantation mouse embryos


ABSTRACT: Pluripotent stem cells provide a platform to interrogate control elements that function to generate all cell types of the body. Despite their utility for modeling development and disease, the relationship of mouse and human pluripotent stem cell states to one another remains largely undefined. We have shown that mouse embryonic stem (ES) cells and epiblast stem cells (EpiSCs) are distinct, pluripotent states isolated from pre- and post-implantation embryos respectively. Human ES cells are different than mouse ES cells and share defining features with EpiSCs, yet are derived from pre-implantation human embryos. Here we show that EpiSCs can be routinely derived from pre-implantation mouse embryos. The pre-implantation-derived EpiSCs exhibit molecular features and functional properties consistent with bona fide EpiSCs. These results provide a simple method for isolating EpiSCs and offer direct insight into the intrinsic and extrinsic mechanisms that regulate the acquisition of distinct pluripotent states. 6 total samples were analyzed. Three pluripotent cell types (mES cells, E3.5 EpiSCs, and E5.5 EpiSCs) were compared with and without treatment of SB431542 for 4 days.

ORGANISM(S): Mus musculus  

SUBMITTER: Paul Tesar  

PROVIDER: E-GEOD-26814 | ArrayExpress | 2011-01-25

SECONDARY ACCESSION(S): GSE26814PRJNA136007

REPOSITORIES: GEO, ArrayExpress

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Publications

Isolation of epiblast stem cells from preimplantation mouse embryos.

Najm Fadi J FJ   Chenoweth Josh G JG   Anderson Philip D PD   Nadeau Joseph H JH   Redline Raymond W RW   McKay Ronald D G RD   Tesar Paul J PJ  

Cell stem cell 20110301 3


Pluripotent stem cells provide a platform to interrogate control elements that function to generate all cell types of the body. Despite their utility for modeling development and disease, the relationship of mouse and human pluripotent stem cell states to one another remains largely undefined. We have shown that mouse embryonic stem (ES) cells and epiblast stem cells (EpiSCs) are distinct, pluripotent states isolated from pre- and post-implantation embryos respectively. Human ES cells are differ  ...[more]

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