Transcriptomics

Dataset Information

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Expression levels in injured vs. non-injured Calu-3 lung epithelial cells


ABSTRACT: Ventilator induced lung injury can lead to serious conditions like ARDS which are associated with a high mortality (around 30%, Stapleton et al., Chest, 2005). We hypothesized that changes of expression levels of different genes would lead us to the identification of critical target genes, which might influence the inflammation and outcome associated with this condition. We used human whole genome U133 Plus 2.0 microarrays to detail the changes of gene expression and identified distinct classes of up-regulated genes during this process. Confluent non strained and strained human Calu-3 cells were selected for RNA extraction and hybridization on Affymetrix microarrays. The experiment was performed with 3 replicates.

ORGANISM(S): Homo sapiens  

SUBMITTER: Holger K Eltzschig  Kelley S Brodsky   Holger K. Eltzschig    

PROVIDER: E-GEOD-27128 | ArrayExpress | 2011-02-12

SECONDARY ACCESSION(S): GSE27128PRJNA137419

REPOSITORIES: GEO, ArrayExpress

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Publications

HIF1A reduces acute lung injury by optimizing carbohydrate metabolism in the alveolar epithelium.

Eckle Tobias T   Brodsky Kelley K   Bonney Megan M   Packard Thomas T   Han Jun J   Borchers Christoph H CH   Mariani Thomas J TJ   Kominsky Douglas J DJ   Mittelbronn Michel M   Eltzschig Holger K HK  

PLoS biology 20130924 9


While acute lung injury (ALI) contributes significantly to critical illness, it resolves spontaneously in many instances. The majority of patients experiencing ALI require mechanical ventilation. Therefore, we hypothesized that mechanical ventilation and concomitant stretch-exposure of pulmonary epithelia could activate endogenous pathways important in lung protection.To examine transcriptional responses during ALI, we exposed pulmonary epithelia to cyclic mechanical stretch conditions--an in vi  ...[more]

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