Transcriptomics

Dataset Information

5

Ethylene and 1-MCP differentially regulate gene expression during onion (Allium cepa L.) sprout suppression


ABSTRACT: Onion is regarded as non-climacteric. In onion, ethylene can suppress sprouting however, the ethylene binding inhibitor, 1-MCP can also suppress sprout growth although it is unknown how ethylene and 1-MCP elicit the same response. In this study, onion bulbs were treated with 10 μL L-1 ethylene or 1 μL L-1 1-MCP individually or in combination for 24 h at 20°C before or after curing (six weeks) at 20 or 28°C then stored at 1°C. Following curing, a subset of these same onion bulbs was stored separately under continuous air or ethylene (10 μL L-1) at 1°C Six treatments were chosen for microarray analysis; four samples were taken before curing immediately after treatment with ethylene or 1-MCP or ethylene and 1-MCP in combination for 24 h at 20°C. The other two samples and two were taken at the end of storage following 6 weeks curing at 28°C andafter 29 weeks cold storage (1°C) in continuous air or continuous ethylene totalling 35 weeks storage. The four pre-curing samples used were untreated (control) or treated with EB, MB or EMB and the two samples after storage were control bulbs stored in continuous ethylene or air at 1°C. There were three biological replicates of each of the six treatments making 18 samples in total.

ORGANISM(S): Allium cepa  

SUBMITTER: Katherine Cools   Gemma A Chope  John Hammond  Andrew J Thompson  John P Hammond  Leon A Terry 

PROVIDER: E-GEOD-27132 | ArrayExpress | 2011-06-29

SECONDARY ACCESSION(S): GSE27132PRJNA137651

REPOSITORIES: GEO, ArrayExpress

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Publications

Regulatory hotspots are associated with plant gene expression under varying soil phosphorus supply in Brassica rapa.

Hammond John P JP   Mayes Sean S   Bowen Helen C HC   Graham Neil S NS   Hayden Rory M RM   Love Christopher G CG   Spracklen William P WP   Wang Jun J   Welham Sue J SJ   White Philip J PJ   King Graham J GJ   Broadley Martin R MR  

Plant physiology 20110428 3


Gene expression is a quantitative trait that can be mapped genetically in structured populations to identify expression quantitative trait loci (eQTL). Genes and regulatory networks underlying complex traits can subsequently be inferred. Using a recently released genome sequence, we have defined cis- and trans-eQTL and their environmental response to low phosphorus (P) availability within a complex plant genome and found hotspots of trans-eQTL within the genome. Interval mapping, using P supply  ...[more]

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