Transcriptomics

Dataset Information

0

CD133+ cells contain cancer stem cell-like properties in Malignant Mixed Mullerian Tumor (Carcinosarcoma) of the Uterus


ABSTRACT: Cancer stem cells (CSCs) that display tumor-initiating properties have recently been identified. We herein identify and characterize CSCs in human uterine carcinosarcoma, a highly aggressive and therapy-resistant gynecologic malignancy, which is considered to be of mesodermal origin. FU-MMT-1, a cell-line, which was established by us (Emoto M, Cancer 1992) from a patient with uterine carcinosarcoma, was evaluated. FU-MMT-1 contained a high population of CD133, CD44, CD90, and CD29 positive cells. Using the magnetic bead cell separation method, we isolated CD133+ cells, which predominantly form spheres in culture. These CD133+ cells form transplantable tumors in vivo. A qRT-PCR analysis of the genes implicated in stem cell maintenance revealed that CD133+ cells express significantly higher levels of OCT4, NANOG, and BMI-1 than CD133- cells. Moreover, CD133+ cells showed a high expression of PAX2 and WNT4, which are the essential genes in Mullerian duct formation. The tumor derived from CD133+ cells replicated vimentin, ERα, ERβ, and PR expressions of the parent tumor. These findings suggest that CD133+ FU-MMT-1 cells have the characteristics of CSCs and Mullerian mesenchymal progenitors. CD133+ and CD133- population of FU-MMT-1 cells were analyzed by microarray.

ORGANISM(S): Homo sapiens  

SUBMITTER: Batsuren Choijamts   Shiro Jimi  Emoto Makoto  Masahide Kuroki  Toru Kondo  Shingo Miyamoto  Yasuko Naganuma  Satoshi Kondo  Hiroshi Iwasaki 

PROVIDER: E-GEOD-27172 | ArrayExpress | 2013-02-10

SECONDARY ACCESSION(S): GSE27172PRJNA137383

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

| GSE27172 | GEO
2015-03-05 | E-GEOD-66529 | ArrayExpress
2016-02-06 | E-GEOD-77612 | ArrayExpress
2016-02-06 | E-GEOD-77613 | ArrayExpress
2016-02-06 | E-GEOD-77614 | ArrayExpress
2016-01-15 | E-GEOD-64854 | ArrayExpress
2011-08-04 | E-GEOD-23456 | ArrayExpress
2011-01-04 | E-GEOD-24747 | ArrayExpress
2008-03-06 | E-MEXP-1412 | ArrayExpress
2014-10-10 | E-MEXP-1412 | ExpressionAtlas