Transcriptomics

Dataset Information

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RNA profiles of total RNA and Ago2-immunoprecipitated RNA


ABSTRACT: Compared gene enrichment in Ago2-RNA immunopreciptant between wildtype and Mir140-null chondrocytes Identified Mir140 targets that are most exclusively regulated by Mir140 Biological duplicate. Total RNA and Ago2-immunoprecipitated RNA were prepared from the same primary chondrocyte culture. Chondrocytes were isolated from newborn mouse rib and cultured overnight in 10%FCS-containing medium. Total RNA and Ago2-immunoprecipitated RNA were amplified using Ovation pico, and subjected to microarray analysis (HT-mouse 430 PM). Two sets of experiments (two samples from wildtype mice for total RNA and Ago2 immunoprecipitated RNA, and two samples from Mir140-null mice for total RNA and Ago2 immunoprecipitated RNA) were performed (a total of 8 chips)

ORGANISM(S): Mus musculus  

SUBMITTER: Tatsuya Kobayashi  

PROVIDER: E-GEOD-27177 | ArrayExpress | 2011-06-01

SECONDARY ACCESSION(S): GSE27177PRJNA137365

REPOSITORIES: GEO, ArrayExpress

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