Genomics,Multiomics

Dataset Information

7

IGH analysis in pro-B cells with H3K4me3, H3K4me2, H3K9ac, H3K27me3, Pax5 and CTCF [ChIP-Seq]

ABSTRACT: VH-DJH recombination of the immunoglobulin heavy-chain (Igh) locus is temporally and spatially controlled during early B-cell development, and yet no regulatory elements other than the VH gene promoters have been identified throughout the entire 2.5-Mb VH gene cluster. Here we discovered novel regulatory sequences that are interspersed in the distal VH gene region. These conserved repeat elements were characterized by the presence of Pax5-dependent active chromatin, the binding of Pax5, E2A, CTCF and Rad21 as well as by Pax5-dependent antisense transcription in pro-B cells. The Pax5-activated intergenic repeat (PAIR) elements were no longer bound by Pax5 in pre-B and B cells consistent with the loss of antisense transcription, whereas E2A and CTCF interacted with PAIR elements throughout early B-cell development. The pro-B-cell-specific and Pax5-dependent activity of the PAIR elements suggests that they are involved in the regulation of distal VH-DJH recombination at the Igh locus. Analysis of chromatin and TF binding in rag2-/- and wt pro-B, DP T and Mature B cells. Chip-Seq of CTCF and Rad21. The provided data is in mm8 coordinates.

OTHER RELATED OMICS DATASETS IN: PRJNA137519PRJNA141973PRJNA141951PRJNA139441

ORGANISM(S): Mus musculus  

SUBMITTER: Markus Jaritz   Maria Novatchkova  Anja Ebert  Shane McManus  Meinrad Busslinger 

PROVIDER: E-GEOD-27214 | ArrayExpress | 2011-02-11

SECONDARY ACCESSION(S): GSE27214SRP005684PRJNA141973

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

The distal V(H) gene cluster of the Igh locus contains distinct regulatory elements with Pax5 transcription factor-dependent activity in pro-B cells.

Ebert Anja A   McManus Shane S   Tagoh Hiromi H   Medvedovic Jasna J   Salvagiotto Giorgia G   Novatchkova Maria M   Tamir Ido I   Sommer Andreas A   Jaritz Markus M   Busslinger Meinrad M  

Immunity 20110201 2

V(H)-DJ(H) recombination of the immunoglobulin heavy chain (Igh) locus is temporally and spatially controlled during early B cell development, and yet no regulatory elements other than the V(H) gene promoters have been identified throughout the entire V(H) gene cluster. Here, we discovered regulatory sequences that are interspersed in the distal V(H) gene region. These conserved repeat elements were characterized by the presence of Pax5 transcription factor-dependent active chromatin by binding  ...[more]

PMID: 21349430

Publication: 1/2

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