Dataset Information


Metabolic control of adult neural stem cell activity by FASN-­dependent lipogenesis

ABSTRACT: Mechanisms controlling the proliferative activity of neural stem/progenitor cells (NSPCs) play a pivotal role to ensure life-long neurogenesis in the mammalian brain. How metabolic programs are coupled with NSPC activity remains unknown. Here we show that fatty acid synthase (FASN), the key enzyme of de novo lipogenesis, is highly active in adult NSPCs and that conditional deletion of FASN in NSPCs impairs adult neurogenesis. The rate of de novo lipid synthesis and subsequent proliferation of NSPCs is regulated by Spot14, a gene we found to be selectively expressed in low proliferating adult NSPCs. Spot14 reduces the availability of malonyl-CoA, which is an essential substrate for FASN to fuel lipogenesis. Thus, we here identified a functional coupling between the regulation of lipid metabolism and adult NSPC proliferation. 6 samples were analyzed. Spot14+: Spot14 GFP positive mouse neural stem cell, 3 biological rep Spot14-: Spot14 GFP negative mouse neural stem cell, 3 biological rep

ORGANISM(S): Mus musculus  

SUBMITTER: Simon M Braun   Clay F Semenkovich  Luis Zurkirchen  Marta Roccio  Nicola Zamboni  Ozlem Karalay  Carolin von Schoultz  Sebastian Jessberger  Marlen Knobloch  Matthias P Lutolf  Werner J Kovacs  Marcos J Araúzo-Bravo  Raquel A Machado  Ueli Suter 

PROVIDER: E-GEOD-27391 | ArrayExpress | 2012-11-02



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