Transcriptomics

Dataset Information

9

Transcriptome partitioning in mouse fertilization.


ABSTRACT: RNA transcripts are distributed non-uniformly in the oocytes of many animals, such that newly-divided embryo cells (blastomeres) inherit distinct transcriptomes following fertilization. In animals such as the frog, Xenopus laevis, programmed transcript regionalization directs early embryonic axis formation and is essential for normal development. However, it is unknown whether such transcriptome asymmetry directs embryogenesis in mammals, or indeed, whether it occurs at all. We here address this by transcript profiling of matching sub-cellular structures and single cells in mouse oocytes and early embryos and analytical strategies exploiting the paired data structure. Spindle samples contained a set of transcripts that was distinguishable from that of the unfertilized metaphase II (mII) oocytes from which each spindle was microsurgically dissected. Immediately following fertilization, cytokinesis produces a 1-cell embryo (zygote) and associated spindle-enriched second polar body (Pb2) whose transcript profiles also differed one from the other, partially reflecting the enrichment of spindle-associated transcripts. Non-uniform transcript distribution within zygotes did not lead to programmed transcriptome asymmetry between the blastomeres of nascent two-cell embryos, or between the second mitotic products of 3-cell embryos. These findings suggest that mammalian oocytes and zygotes exhibit transcript regionalization without subsequent transcriptome asymmetries between respective early mitotic products. This contrasts the situation in Xenopus and places constraints on the ability of maternal transcriptomic prepatterning to prescribe early mammalian development. 16 Zygote vs. Polar body pairs, 10 spindle vs. oocyte pairs, 22 2-cell embryos and 8 3-cell embryos were analysed. Although the raw data are two channel, only the Cy5 signal of each file was analyzed. The Cy5 channel for each gene is normalized to the average Cy5 intensity of the gene across all samples.

ORGANISM(S): Mus musculus  

SUBMITTER: Isabell Blochberger   Toru Suzuki  Christoph A Klein  Matthias Maneck  Anthony C Perry  Emi Suzuki  Naoko Yoshida  Matthew D VerMilyea  Rainer Spang 

PROVIDER: E-GEOD-27396 | ArrayExpress | 2011-04-21

SECONDARY ACCESSION(S): GSE27396PRJNA136999

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptome asymmetry within mouse zygotes but not between early embryonic sister blastomeres.

VerMilyea Matthew D MD   Maneck Matthias M   Yoshida Naoko N   Blochberger Isabell I   Suzuki Emi E   Suzuki Toru T   Spang Rainer R   Klein Christoph A CA   Perry Anthony C F AC  

The EMBO journal 20110405 9


Transcriptome regionalization is an essential polarity determinant among metazoans, directing embryonic axis formation during normal development. Although conservation of this principle in mammals is assumed, recent evidence is conflicting and it is not known whether transcriptome asymmetries exist within unfertilized mammalian eggs or between the respective cleavage products of early embryonic divisions. We here address this by comparing transcriptome profiles of paired single cells and sub-cel  ...[more]

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