Transcriptomics

Dataset Information

2

Transcriptome analysis of constitutive resistance to white pine weevil in Picea glauca-engelmannii (Interior spruce)


ABSTRACT: White pine weevil is a major pest of conifers in North America, especially for Spruce trees. Constitutive defenses are important in understanding defense mechanisms because they constitute the initial barrier to attacks by weevils and other pests. Resistant and susceptible trees exhibit constitutive differences in spruce. To improve our knowledge of their genetic basis, we compared the constitutive expression levels of 17,825 genes between 20 resistant and 20 susceptible trees in interior spruce (Picea glauca). Twenty hybridizations were performed to compare untreated bark of resistant and susceptible trees.RNA isolated from each of the 20 individual untreated resistant trees was compared directly against the 20 individual untreated susceptble trees using two hybridizations with a dye flip for each tree pair.

ORGANISM(S): Picea engelmannii x Picea glauca  

SUBMITTER: Sébastien Verne   Rick White  Carol Ritland  Kermit Ritland  Barry Jaquish 

PROVIDER: E-GEOD-27476 | ArrayExpress | 2011-11-28

SECONDARY ACCESSION(S): GSE27476PRJNA138431

REPOSITORIES: GEO, ArrayExpress

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Publications

Global transcriptome analysis of constitutive resistance to the white pine weevil in spruce.

Verne Sébastien S   Jaquish Barry B   White Rick R   Ritland Carol C   Ritland Kermit K  

Genome biology and evolution 20110817


Constitutive defense mechanisms are critical to the understanding of defense mechanisms in conifers because they constitute the first barrier to attacks by insect pests. In interior spruce, trees that are putatively resistant and susceptible to attacks by white pine weevil (Pissodes strobi) typically exhibit constitutive differences in traits such as resin duct size and number, bark thickness, and terpene content. To improve our knowledge of their genetic basis, we compared globally the constitu  ...[more]

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