Transcriptomics

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Gene expression and epigenetic profiling of CD34+ hematopoietic progenitor cells in multiple sclerosis patients (miRNA)


ABSTRACT: Intense immunosuppression followed by autologous hematopoietic stem cell transplantation (aHSCT) is a potential treatment for patients suffering from aggressive multiple sclerosis (MS). However it remains unresolved whether autologous CD34+ hematopoietic progenitor cells of MS patients show gene expression differences prior to aHSCT that indicate a preset proinflammatory state, which would then also predispose to or predetermine recurrence of the autoimmune disease. To approach this key point we compared the micro RNA gene expression signature of CD34+ cells collected from MS patients and healthy donors (HD). Gene expression of CD34+ cells was analysed with the Human miRNA Microarray (Agilent-Technologies) chip. No substantial alterations in the gene expression profile of CD34+ HPCs in MS were observed. Samples of CD34+ cells were obtained from 4 female MS patients and 4 age matched healthy donors (3 female) mobilized by G-CSF (2x5μg/kg/day). White blood cells, containing the CD34+ cell fraction, were collected by leucocytapheresis from peripheral blood, frozen and stored in liquid nitrogen. All samples were thawed and processed at one center and CD34+ HPCs purified by magnetic bead separation using the autoMACS system (Miltenyi). Purity and viability of CD34+ cells was analyzed by Fluorescence Activated Cell Sorter (FACS). Total cellular RNA were extracted with TRIzol reagent and analyzed with the Human miRNA Microarray (Agilent-Technologies).

ORGANISM(S): Homo sapiens  

SUBMITTER: Christian Schulze   Mireia Sospedra  Annamaria Repice  Nicolaus Kroeger  Gabriela Salinas-Riester  Axel Zander  Benedetta Mazzanti  Andreas Lutterotti  Petra Breiden  Andreas Sputtek  Stefanie Reinhard  Massimo DiGioia  Luca Massacesi  Riccardo Saccardi  Roland Martin  Sven Schippling  Ilijas Jelcic 

PROVIDER: E-GEOD-27690 | ArrayExpress | 2012-09-05

SECONDARY ACCESSION(S): GSE27690PRJNA141873

REPOSITORIES: GEO, ArrayExpress

Dataset's files

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Action DRS
E-GEOD-27690.idf.txt Idf
E-GEOD-27690.processed.1.zip Processed
E-GEOD-27690.raw.1.zip Raw
E-GEOD-27690.sdrf.txt Txt
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Publications

No proinflammatory signature in CD34+ hematopoietic progenitor cells in multiple sclerosis patients.

Lutterotti A A   Jelcić I I   Schulze C C   Schippling S S   Breiden P P   Mazzanti B B   Reinhardt S S   DiGioia M M   Repice A A   Massacesi L L   Sputtek A A   Salinas-Riester G G   Kroeger N N   Sospedra M M   Saccardi R R   Zander A A   Martin R R  

Multiple sclerosis (Houndmills, Basingstoke, England) 20120117 8


Autologous hematopoietic stem cell transplantation (aHSCT) has been used as a therapeutic approach in multiple sclerosis (MS). However, it is still unclear if the immune system that emerges from autologous CD34+ hematopoietic progenitor cells (HPC) of MS patients is pre-conditioned to re-develop the proinflammatory phenotype. The objective of this article is to compare the whole genome gene and microRNA expression signature in CD34+ HPC of MS patients and healthy donors (HD). CD34+ HPC were isol  ...[more]

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