Transcriptomics

Dataset Information

3

Identification of differentially regulated genes upon overexpression of HLX in wild-type hematopoietic stem cells


ABSTRACT: The goal was to study the role of Hlx in hematopoiesis. Sorted Lin-Kit+Sca-1+ cells from wild-type FVB/nJ bone marrow were infected with control (pCAD-IRES-GFP) or Hlx lentivirus (pCAD-IRES-GFP-Hlx) and cultured for 2 days in Iscove’s modified Dulbecco’s medium (IMDM) containing FBS, mIL-3, mIL-6 and mSCF with lentiviral supernatants in the presence of 8ug/ml polybrene. Subsequently, GFP+ cells were sorted by FACS and RNA was prepared. Three replicates of each, control vector transduced and HLX-transduced cells were used.

ORGANISM(S): Mus musculus  

SUBMITTER: Boris Bartholdy   Masahiro Kawahara  Britta Will  Ulrich G Steidl  Laura Barreyro  Constantine S Mitsiades 

PROVIDER: E-GEOD-27938 | ArrayExpress | 2012-09-14

SECONDARY ACCESSION(S): GSE27938PRJNA141719

REPOSITORIES: GEO, ArrayExpress

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Publications


Homeobox domain-containing transcription factors are important regulators of hematopoiesis. Here, we report that increased levels of nonclustered H2.0-like homeobox (HLX) lead to loss of functional hematopoietic stem cells and formation of aberrant progenitors with unlimited serial clonogenicity and blocked differentiation. Inhibition of HLX reduces proliferation and clonogenicity of leukemia cells, overcomes the differentiation block, and leads to prolonged survival. HLX regulates a transcripti  ...[more]

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