Name: Transcription profiling by array of immature ovariectomized mice after treatment with 17-alpha-ethynylestradiol
Keywords: transcription profiling by array

Dataset Information

Transcription profiling by array of immature ovariectomized mice after treatment with 17-alpha-ethynylestradiol

ABSTRACT: Immature, ovariectomized C57BL/6 mice were treated with sesame oil (vehicle) or with 0.1 mg/kg 17-alpha-ethynylestradiol (EE) for the length of time indicated. Mice received one dose, except for the 3x24hr samples, which received 3 consecutive daily doses and were sacrificed 24hr after the final dose. Two replicates of each sample are provided. In addition, one time=0 sample is included.

OTHER RELATED OMICS DATASETS IN: PRJNA84717

ORGANISM(S): Mus Musculus

SUBMITTER: Tim Zacharewski

PROVIDER: E-GEOD-280 | ArrayExpress | 2007-12-13

SECONDARY ACCESSION(S): GDS285GSE280PRJNA84717

REPOSITORIES: GEO, ArrayExpress

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Publications

Identification of temporal patterns of gene expression in the uteri of immature, ovariectomized mice following exposure to ethynylestradiol.

Fertuck K C KC Eckel J E JE Gennings C C Zacharewski T R TR

Physiological genomics 20031017 2

Estrogen induction of uterine wet weight provides an excellent model to investigate relationships between changes in global gene expression and well-characterized physiological responses. In this study, time course microarray GeneChip data were analyzed using a novel approach to identify temporal changes in uterine gene expression following treatment of immature ovariectomized C57BL/6 mice with 0.1 mg/kg 17alpha-ethynylestradiol. Functional gene annotation information from public databases facil ...[more]

PMID: 12915738

Similar Datasets

Project description:<h4>Background</h4>Uterine temporal and dose-dependent histopathologic, morphometric and gene expression responses to the selective estrogen receptor modulator tamoxifen (TAM) were comprehensively examined to further elucidate its estrogen receptor-mediated effects. These results were systematically compared to the effects elicited by the potent estrogen receptor ligand 17alpha-ethynylestradiol (EE) to identify pathways similarly and uniquely modified by each compound.<h4>Results</h4>Three daily doses of 100 microg/kg TAM elicited a dose-dependent increase in uterine wet weight (UWW) in immature, ovariectomized C57BL/6 mice at 72 hrs with concurrent increases in luminal epithelial cell height (LECH), luminal circumference and glandular epithelial tubule number. Significant UWW and LECH increases were detected at 24 hrs after a single dose of 100 microg/kg TAM. cDNA microarray analysis identified 2235 differentially expressed genes following a single dose of 100 microg/kg TAM at 2, 4, 8, 12, 18 and 24 hrs, and at 72 hrs after three daily doses (3 x 24 hrs). Functional annotation of differentially expressed genes was associated with cell growth and proliferation, cytoskeletal organization, extracellular matrix modification, nucleotide synthesis, DNA replication, protein synthesis and turnover, lipid metabolism, glycolysis and immunological responses as is expected from the uterotrophic response. Comparative analysis of TAM and EE treatments identified 1209 common, differentially expressed genes, the majority of which exhibited similar profiles despite a temporal delay in TAM elicited responses. However, several conserved and treatment specific responses were identified that are consistent with proliferation (Fos, Cdkn1a, Anapc1), and water imbibition (Slc30a3, Slc30a5) responses elicited by EE.<h4>Conclusion</h4>Overall, TAM and EE share similar gene expression profiles. However, TAM responses exhibit lower efficacy, while responses unique to EE are consistent with the physiological differences elicited between compounds.

			Action	DRS
	E-GEOD-280.README.txt	Txt
	E-GEOD-280.idf.txt	Idf
	E-GEOD-280.processed.1.zip	Processed
	E-GEOD-280.sdrf.txt	Txt