Transcriptomics

Dataset Information

6

Role of enterotoxins in the induction of early immune responses and small-intestinal secretion in ETEC-infected piglets


ABSTRACT: Enterotoxigenic Escherichia coli (ETEC) strains that produce both heat-stable (ST) and heat-labile (LT) enterotoxins cause severe post-weaning diarrhea in piglets. However, the relative importance of the individual enterotoxins to the pathogenesis of ETEC infection is poorly understood. In this study, we investigated the effect on virulence of an F4+ ETEC strain when removing some or all of its enterotoxins. Several isogenic mutant strains were constructed that lack the expression of LT in combination with one or both types of ST enterotoxins (STa and/or STb). Host early immune responses induced by these mutant strains 4h after infection were compared to the wild type strain GIS26  (O149:F4ac+,  LT+  STa+  STb+). At the same time, the immune response of this wild type ETEC strain was compared to the mock-infected control, demonstrating the expression of porcine inflammatory response genes. For these purposes, the small intestinal segment perfusion (SISP) technique and microarray analysis were used and results were validated by qRT-PCR. We also measured net fluid absorption of pig small intestinal mucosa 4h after infection with wild type ETEC, the mutant strains and PBS (mock-infected). These data indicate an important role for STb in inducing small intestinal secretion early after infection. The microarray analysis of the different mutant strains also revealed an important role for STb in ETEC-induced immune response by the significant differential regulation of immune mediators like matrix metalloproteinase 3, interleukin 1 and interleukin 17. We conclude that STb can play a prominent role in ETEC-induced secretion and early immune response. In three pigs, 6 different treatments were performed. These treatments consisted of 4 mutant enterotoxigenic Escherichia coli GIS26 strains, GIS26 wild type strain, or PBS control. Per pig, the small intestine was divided into 6 loops with an interloop in between to avoid cross-contamination. In conclusion, every pig received each of the 6 treatments ad random.

ORGANISM(S): Sus scrofa  

SUBMITTER: Theo Niewold   Joke Allemeersch  Michaela Loos  Frank Gasthuys  Eric Cox  Marisa Geens  Bruno Goddeeris  Stijn Schauvliege 

PROVIDER: E-GEOD-28003 | ArrayExpress | 2013-01-02

SECONDARY ACCESSION(S): GSE28003PRJNA137785

REPOSITORIES: GEO, ArrayExpress

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Publications

Role of heat-stable enterotoxins in the induction of early immune responses in piglets after infection with enterotoxigenic Escherichia coli.

Loos Michaela M   Geens Marisa M   Schauvliege Stijn S   Gasthuys Frank F   van der Meulen Jan J   Dubreuil J Daniel JD   Goddeeris Bruno M BM   Niewold Theo T   Cox Eric E  

PloS one 20120717 7


Enterotoxigenic Escherichia coli (ETEC) strains that produce heat-stable (ST) and/or heat-labile (LT) enterotoxins are cause of post-weaning diarrhea in piglets. However, the relative importance of the different enterotoxins in host immune responses against ETEC infection has been poorly defined. In the present study, several isogenic mutant strains of an O149:F4ac(+), LT(+) STa(+) STb(+) ETEC strain were constructed that lack the expression of LT in combination with one or both types of ST ente  ...[more]

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