Transcriptomics

Dataset Information

2

Expression profiles of mouse glioma-initiating cells.


ABSTRACT: To identify factors involved in glioma-initiating cells (GICs), we compared gene expressions between GIC-like cells and non-GICs. p53-deficient Neural stem cells (NSCs), oligodendrocyte precursor cells (OPCs), and astrocytes (ASTs) were transfected with pCMS-EGFP-HRasL61 and pBabe-neo by electroporation and cultured in 0.5 mg/ml neomycin. The GFP-positive stable NSCs (NSCL61s), OPCs (OPCL61s) and ASTs (ASTL61s) were purified by flow cytometry. Total RNA was prepared using a RNeasy Mini Kit (QIAGEN) and used for the expression analysis. 

ORGANISM(S): Mus musculus  

SUBMITTER: Toru Kondo  

PROVIDER: E-GEOD-28091 | ArrayExpress | 2011-06-01

SECONDARY ACCESSION(S): GSE28091PRJNA139747

REPOSITORIES: GEO, ArrayExpress

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Publications

Combination of a ptgs2 inhibitor and an epidermal growth factor receptor-signaling inhibitor prevents tumorigenesis of oligodendrocyte lineage-derived glioma-initiating cells.

Hide Takuichiro T   Takezaki Tatsuya T   Nakatani Yuka Y   Nakamura Hideo H   Kuratsu Jun-ichi J   Kondo Toru T  

Stem cells (Dayton, Ohio) 20110401 4


Recent findings have demonstrated that malignant tumors, including glioblastoma multiforme (GBM), contain cancer-initiating cells (CICs; also known as cancer stem cells), which self-renew and are malignant. However, it remains controversial whether such CICs arise from tissue-specific stem cells, committed precursor cells, or differentiated cells. Here, we sought to examine the origin of the CICs in GBM. We first showed that the overexpression of oncogenic HRas(L61) transformed p53-deficient oli  ...[more]

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