Transcriptomics

Dataset Information

45

PRMT1 interacts with leukemia fusion protein AML1-ETO for activation of transcription and transformation


ABSTRACT: Fusion protein AML1-ETO resulted from t(8;21) translocation is highly related to leukemia development. We have previously shown that the expression of AE9a, a spliced form of AML1-ETO, can rapidly cause leukemia in mouse. To understand how AML1-ETO is involved in leukemia development, we used AE9a leukemia model to identify a novel AE9a interacting proteins PRMT1 (protein arginine methyltransferase 1) from primary leukemic cells expressing AE9a. To examine whether PRMT1 is involved in AE9a-mediated transcription regulation, genome wide gene expression analysis is carried out in hematopoietic cell line K562 (wild type or AE9a expressing) treated with (-) control siRNA or siPRMT1. Wild type or AE9a-expressing K562 cells with control siRNA or siPRMT1 in triplicate

ORGANISM(S): Homo sapiens  

SUBMITTER: Shinobu Matsuura  Ming Yan   Stephen D Nimer   Wei-Jong Shia   John R Yates III   Dong-Er Zhang   Ali Sarkeshik   Xinyang Zhao   Miao-Chia Lo   Akiko J Okumura    

PROVIDER: E-GEOD-28135 | ArrayExpress| 2012-06-26

SECONDARY ACCESSION(S): GSE28135PRJNA139549

REPOSITORIES: GEO, ArrayExpress

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Publications

PRMT1 interacts with AML1-ETO to promote its transcriptional activation and progenitor cell proliferative potential.

Shia Wei-Jong WJ   Okumura Akiko J AJ   Yan Ming M   Sarkeshik Ali A   Lo Miao-Chia MC   Matsuura Shinobu S   Komeno Yukiko Y   Zhao Xinyang X   Nimer Stephen D SD   Yates John R JR   Zhang Dong-Er DE  

Blood 20120412 21


Fusion protein AML1-ETO, resulting from t(8;21) translocation, is highly related to leukemia development. It has been reported that full-length AML1-ETO blocks AML1 function and requires additional mutagenic events to promote leukemia. We have previously shown that the expression of AE9a, a splice isoform of AML1-ETO, can rapidly cause leukemia in mice. To understand how AML1-ETO is involved in leukemia development, we took advantage of our AE9a leukemia model and sought to identify its interact  ...[more]

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