Transcriptomics,Multiomics

Dataset Information

8

Transcription profiling of human MCF7 cells infected with adenovirus expressing c-Myb, v-Myb or the hybrid construct, 3MutC


ABSTRACT: The transcriptional activities of c-Myb and its oncogenic variant v-Myb were compared by expressing them in human MCF7 cells using recombinant adenovirus vectors. A hybrid construct, 3Mutc, which is a variant of c-Myb harboring three v-Myb-derived DNA binding domain mutations was also analyzed. All the samples were compared to cells infected with a control adenovirus. The results showed that v-Myb, which differs from c-Myb only by N- and C-terminal deletions and eleven amino acid substitutions, has a qualitatively different transcriptional activity. Experiment Overall Design: 2 replicates of each type were analyzed. Replicates were performed independently, more than a week apart.

OTHER RELATED OMICS DATASETS IN: PRJNA92419PRJNA92417

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo Sapiens

SUBMITTER: Scott A. Ness  

PROVIDER: E-GEOD-2815 | ArrayExpress | 2007-12-12

SECONDARY ACCESSION(S): GSE2815GDS1384PRJNA92417

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-2815.README.txt Txt
E-GEOD-2815.eSet.r Other
E-GEOD-2815.idf.txt Idf
E-GEOD-2815.processed.1.zip Processed
E-GEOD-2815.raw.1.zip Raw
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Publications

Oncogenic mutations cause dramatic, qualitative changes in the transcriptional activity of c-Myb.

Liu F F   Lei W W   O'Rourke J P JP   Ness S A SA  

Oncogene 20060201 5


The v-Myb oncoprotein encoded by Avian Myeloblastosis Virus is highly oncogenic, induces leukemias in chickens and mice and transforms immature hematopoietic cells in vitro. The v-Myb protein is a mutated and truncated version of c-Myb, a DNA-binding transcription factor expressed in many cell types that is essential for normal hematopoiesis. Previous studies suggested that two types of differences, DNA binding domain mutations and the deletion of a C-terminal negative regulatory domain were imp  ...[more]

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