Transcriptomics

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Transcription profiling of human monocytes infected with adenovirus expressing either c-Myb or v-Myb


ABSTRACT: The transcriptional activities of c-Myb and its oncogenic variant v-Myb were compared by expressing them in primary human monocytes using recombinant adenovirus vectors. All the samples were compared to cells infected with a control adenovirus expressing only GFP. The results showed that v-Myb, which differs from c-Myb only by N- and C-terminal deletions and eleven amino acid substitutions, has a qualitatively different transcriptional activity. Experiment Overall Design: 2 replicates of each type were analyzed. Replicates were performed independently, more than a week apart.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Scott A. Ness  

PROVIDER: E-GEOD-2816 | ArrayExpress | 2007-12-12

SECONDARY ACCESSION(S): GDS1427GSE2816PRJNA92419

REPOSITORIES: GEO, ArrayExpress

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Publications

Oncogenic mutations cause dramatic, qualitative changes in the transcriptional activity of c-Myb.

Liu F F   Lei W W   O'Rourke J P JP   Ness S A SA  

Oncogene 20060201 5


The v-Myb oncoprotein encoded by Avian Myeloblastosis Virus is highly oncogenic, induces leukemias in chickens and mice and transforms immature hematopoietic cells in vitro. The v-Myb protein is a mutated and truncated version of c-Myb, a DNA-binding transcription factor expressed in many cell types that is essential for normal hematopoiesis. Previous studies suggested that two types of differences, DNA binding domain mutations and the deletion of a C-terminal negative regulatory domain were imp  ...[more]

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