Transcriptomics

Dataset Information

100

Two-step RNA sequencing identifies a tissue-specific landscape of sense/antisense transcription


ABSTRACT: We report the complexity of the polyadenylated transcriptome of the mouse intestinal tract assessed by two step RNA sequencing. Screening and verification of non-annotated transcriptional units by different RNA-Seq protocols.

ORGANISM(S): Mus musculus  

SUBMITTER: Ulrich Klostermeier  Robert Haesler   Philip Rosenstiel    

PROVIDER: E-GEOD-28207 | ArrayExpress | 2014-05-02

SECONDARY ACCESSION(S): SRP006204GSE28207PRJNA139649

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications


BACKGROUND: The intestinal mucosa is characterized by complex metabolic and immunological processes driven highly dynamic gene expression programs. With the advent of next generation sequencing and its utilization for the analysis of the RNA sequence space, the level of detail on the global architecture of the transcriptome reached a new order of magnitude compared to microarrays. RESULTS: We report the ultra-deep characterization of the polyadenylated transcriptome in two closely related, yet d  ...[more]

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