Transcriptomics

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DNA methylation analysis of normal and cancer cells


ABSTRACT: DNA methylation of prostatic normal cells (PrEC), normal mammary epithelial cells (HMEC), prostate cancer cell line (MDA-PCa-2b), and breast cancer cell lines (BT474 and MDA-MB-231) was analyzed by MeDIP-on-chip analysis. DNA obtained from each cells was immunoprecipitated by anti 5-methylcytidine antibody. IP DNA and input DNA were labeled with Cy5 and Cy3, respectively, and then analyzed by using human CpG island microarray provided by Agilent Technologies.

ORGANISM(S): Homo sapiens  

SUBMITTER: Hideyuki Takeshima   

PROVIDER: E-GEOD-28284 | ArrayExpress | 2011-06-06

SECONDARY ACCESSION(S): GSE28284PRJNA139361

REPOSITORIES: GEO, ArrayExpress

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