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A single functional blast resistance gene Pi54 activates complex defense mechanism in Rice

ABSTRACT: To investigate the responses of resistance gene Pi54 at transcriptome level in blast resistant transgenic rice line TP-Pi54 during infection by Magnaporthe oryzae, we studied the co-expression analysis using microarray along with susceptible non-transgenic line Taipei 309 (TP). Suitable controls for both lines were used. The comparative analysis of differentially expressed genes was conducted. Total RNA from the leaves of mock inoculated non transgenic line Taipei 309 (TP) and transgenic line (TP-Pi54) along with challenge inoculated plants were isolated at 72 hours post inoculation. The samples were analyzed in two different biological replications (A and B) making total eight samples.

ORGANISM(S): Oryza sativa Japonica Group  

SUBMITTER: Shamsher S Kanwar   Dr. Tilak Raj Sharma  Tilak R Sharma  Duni Chand  Santosh K Gupta  Nagendra K Singh  Amit K Rai 

PROVIDER: E-GEOD-28308 | ArrayExpress | 2011-12-08



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The single functional blast resistance gene Pi54 activates a complex defence mechanism in rice.

Gupta Santosh Kumar SK   Rai Amit Kumar AK   Kanwar Shamsher Singh SS   Chand Duni D   Singh Nagendera Kumar NK   Sharma Tilak Raj TR  

Journal of experimental botany 20111104 2

The Pi54 gene (Pi-k(h)) confers a high degree of resistance to diverse strains of the fungus Magnaporthe oryzae. In order to understand the genome-wide co-expression of genes in the transgenic rice plant Taipei 309 (TP) containing the Pi54 gene, microarray analysis was performed at 72 h post-inoculation of the M. oryzae strain PLP-1. A total of 1154 differentially expressing genes were identified in TP-Pi54 plants. Of these, 587 were up-regulated, whereas 567 genes were found to be down-regulate  ...[more]

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