Transcriptomics

Dataset Information

4

Expression data from mouse small intestinal γδ intraepithelial lymphocytes


ABSTRACT: The mammalian gastrointestinal tract harbors thousands of bacterial species that include symbionts as well as potential pathogens. The immune responses that limit access of these bacteria to underlying tissue remain poorly defined. In this study, we used microarrays to uncover the transcriptional responses that occur in small intestinal γδ intraepithelial lymphocytes following bacterial challenge. γδ intraepithelial lymphocytes (γδ IEL) were isolated by flow cytometry from the small intestines of germ-free mice, or from age- and sex-matched conventionally-raised counterparts. We extracted RNAs from these purified γδ IEL for analysis on Affymetrix DNA microarrays. The mice were all >8 weeks in age, and each sample represents a pool of RNAs from 5-8 mice.

ORGANISM(S): Mus musculus  

SUBMITTER: Lora V Hooper   Anisa S Ismail 

PROVIDER: E-GEOD-28437 | ArrayExpress | 2011-04-06

SECONDARY ACCESSION(S): GSE28437PRJNA139243

REPOSITORIES: GEO, ArrayExpress

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Publications

Gammadelta intraepithelial lymphocytes are essential mediators of host-microbial homeostasis at the intestinal mucosal surface.

Ismail Anisa S AS   Severson Kari M KM   Vaishnava Shipra S   Behrendt Cassie L CL   Yu Xiaofei X   Benjamin Jamaal L JL   Ruhn Kelly A KA   Hou Baidong B   DeFranco Anthony L AL   Yarovinsky Felix F   Hooper Lora V LV  

Proceedings of the National Academy of Sciences of the United States of America 20110509 21


The mammalian gastrointestinal tract harbors thousands of bacterial species that include symbionts as well as potential pathogens. The immune responses that limit access of these bacteria to underlying tissue remain poorly defined. Here we show that γδ intraepithelial lymphocytes (γδ IEL) of the small intestine produce innate antimicrobial factors in response to resident bacterial "pathobionts" that penetrate the intestinal epithelium. γδ IEL activation was dependent on epithelial cell-intrinsic  ...[more]

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