Dataset Information


NMLF response to spinal cord injury in Zebrafish

ABSTRACT: The study was designed to identify genes regulated after spinal transection that might contribute to regenerative growth of neurons projecting from the NMLF in Zebrafish. Zebrafish were injured by surgical transection of the spinal cord at 1 mm caudal to the brainstem-spinal cord junction (Injured). Animals receiving sham surgery (identical surgical procedures without transection) served as control (Control). The nucleus of the medial longitudinal fascicle (NMLF) was laser capture microdissected from approximately 30 frozen sections. RNA was prepared, amplified, and run on Affymetrix Zebrafish arrays. Zebrafish were used because they recover swimming function after spinal transection in about 6 weeks. The NMLF has been identified as a prominent group of neurons that descend through the site of injury in the spinal cord and that regenerate after injury. Times were selected to distinguish early events from those in the timeframe of regenerative growth.


ORGANISM(S): Danio rerio  

SUBMITTER: Liping Ma   Melitta Schachner  Ronald P. Hart  Ronald P Hart 

PROVIDER: E-GEOD-28470 | ArrayExpress | 2011-12-01



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Cysteine- and glycine-rich protein 1a is involved in spinal cord regeneration in adult zebrafish.

Ma Liping L   Yu Young-Mi YM   Guo Yuji Y   Hart Ronald P RP   Schachner Melitta M  

The European journal of neuroscience 20120201 3

In contrast to mammals, adult zebrafish have the ability to regrow descending axons and gain locomotor recovery after spinal cord injury (SCI). In zebrafish, a decisive factor for successful spinal cord regeneration is the inherent ability of some neurons to regrow their axons via (re)expressing growth-associated genes during the regeneration period. The nucleus of the medial longitudinal fascicle (NMLF) is one of the nuclei capable of regenerative response after SCI. Using microarray analysis w  ...[more]

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