Dataset Information

Gene expression data from yeast exposure to alachlor

ABSTRACT: The world-wide used herbicide alachlor is among the priority substances listed in the European Water Framework Directive. We aimed at finding molecular biomarkers in the model yeast Saccharomyces cerevisiae that may be used to predict potential cytotoxic effects of this xenobiotic while providing mechanistic clues possibly relevant for experimentally less accessible non-target eukaryotes. We used microarrays to carry out a gene expression profiling analysis in S. cereviseae strain BY4741 upon 2 hours exposure to alachlor at concentrations exerting slight to moderate levels of phenotypic effects (inhibition of yeast growth rate). Two exposure scenarios were analysed, namely the lowest observed effect concentration (LOEC; ~8% growth inhibition) and the 20%-inhibitory concentration (IC20), compared to control cells not exposed to the herbicide (CT02). Exponential standardized cell suspensions of S. cerevisiae BY4741 in minimal growth medium were incubated in the presence of 110 or 200 mg/L of ALA (corresponding to the LOEC and the IC20, respectively, of alachlor) or in medium non-supplemented with the herbicide (control cells, CT02) during 2 h, and used for total RNA isolation and hybridization on Affymetrix microarrays. Exposure experiments with each concentration of alachlor versus controls were carried out independently. For each exposure condition, independent biological triplicates were processed and analysed.

OTHER RELATED OMICS DATASETS IN: PRJNA138931

ORGANISM(S): Saccharomyces Cerevisiae

SUBMITTER: Fátima N Gil Cristina Anjinho Viegas Jörg D Becker Cristina A Viegas

PROVIDER: E-GEOD-28677 | ArrayExpress | 2011-08-27

SECONDARY ACCESSION(S): GSE28677PRJNA138931

REPOSITORIES: GEO, ArrayExpress

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Transcriptional profiling in Saccharomyces cerevisiae relevant for predicting alachlor mechanisms of toxicity.

Gil Fátima N FN Gonçalves Alina C AC Jacinto Maria João MJ Becker Jörg D JD Viegas Cristina A CA

Environmental toxicology and chemistry 20110919 11

Alachlor has been a commonly applied herbicide and is a substance of ecotoxicological concern. The present study aims to identify molecular biomarkers in the eukaryotic model Saccharomyces cerevisiae that can be used to predict potential cytotoxic effects of alachlor, while providing new mechanistic clues with possible relevance for experimentally less accessible eukaryotes. It focuses on genome-wide expression profiling in a yeast population in response to two exposure scenarios exerting effect ...[more]

PMID: 21842488

Similar Datasets

Project description:This study focus a comparative toxicogenomic analysis of the effects of four herbicides (alachlor, ALA, S-metolachlor, S-MET, diuron, DIU, and MCPA-methyl ester, MCPA-ME), one insecticide (carbofuran, CAB), and one fungicide (pyrimethanil, PYR), in the model yeast Saccharomyces cerevisiae, to predict potential cytotoxic effects of these xenobiotics while providing mechanistic clues possibly relevant for experimentally less accessible non-target eukaryotes. The six model pesticides selected have been used worldwide in agricultural activities, at the present time or in the past, and have different modes of action on their target-organisms. Moreover, some of them are currently in Annex 1 of EC Directive 1107/2009 (repealing 91/414), that is they are in use in the EU, but having some ecotoxicological concerns (e.g. S-MET, PYR, MCPA-ME), others have their use restricted and/or are priority substances under the Water Framework Directive (e.g. ALA, DIU), and one was banned (e.g. CAB). We used genome microarrays to obtain gene expression profiles in S. cerevisiae strain BY4741 upon 2 hours exposure to pesticide concentrations exerting a moderate inhibition of yeast growth rate. To obtain comparable gene expression profiles representing yeast response to the six different pesticides, all exposure treatments were performed with equitoxic pesticide concentrations that generated equivalent responses at the phenotypic level (namely, 20% growth-inhibitory concentrations, hereafter designated as the pesticide IC20). Overall design: Exponential standardized cell suspensions of S. cerevisiae BY4741 in minimal growth medium were incubated in the presence of equitoxic concentrations of the pesticides (i.e. the respective IC20), or in medium non-supplemented with the pesticide (control cells, CT02) during 2 h, and used for total RNA isolation and hybridization on Affymetrix GeneChip Yeast Genome 2.0 Arrays. Exposure experiments with the IC20 concentration of each pesticide versus CT02 cells were carried out together. For each exposure condition, independent biological triplicates were processed and analyzed.

			Action	DRS
	E-GEOD-28677.README.txt	Txt
	E-GEOD-28677.eSet.r	Other
	E-GEOD-28677.idf.txt	Idf
	E-GEOD-28677.processed.1.zip	Processed
	E-GEOD-28677.raw.1.zip	Raw