Transcriptomics

Dataset Information

6

Gene expression profiling of imatinib-treated and untreated human Hematopoetic progenitors expressing the ETV6-PDGFRB oncogene


ABSTRACT: In order to investigate the mechanism whereby TEL-PDGF-beta (ETV6-PDGFRB) interferes with human hematopoietic progenitors proliferation and differentiation, we analyzed the gene expression response downstream this oncogene. CD34+ cells infected with lentivirus coding for TEL-PDGFRb were cultured for 7 days in the absence of cytokines. Using Affymetrix microarrays, we compared gene expression in these cells and in cells treated for 4 h with low dose imatinib (Glivec), a potent PDGFR inhibitor, to switch off TEL-PDGFRb signaling. This experiment was performed in three biological replicates. In each replicate gene expression profiling from CD34+ HSCs expressing TEL-PDGFRb untreated with Glivec was compared to the corresoniding treated condition. Total RNA were extracted from transduced CD34+ cells using Trizol reagent (Invitrogen) and the RNeasy kit (QIAGEN).

ORGANISM(S): Homo sapiens  

SUBMITTER: Ahmed Essaghir   Jean-Baptiste Demoulin  Carmen P Montano-Almendras 

PROVIDER: E-GEOD-28698 | ArrayExpress | 2012-04-09

SECONDARY ACCESSION(S): GSE28698PRJNA138911

REPOSITORIES: GEO, ArrayExpress

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Publications

ETV6-PDGFRB and FIP1L1-PDGFRA stimulate human hematopoietic progenitor cell proliferation and differentiation into eosinophils: the role of nuclear factor-κB.

Montano-Almendras Carmen P CP   Essaghir Ahmed A   Schoemans Hélène H   Varis Inci I   Noël Laura A LA   Velghe Amélie I AI   Latinne Dominique D   Knoops Laurent L   Demoulin Jean-Baptiste JB  

Haematologica 20120122 7


ETV6-PDGFRB (also called TEL-PDGFRB) and FIP1L1-PDGFRA are receptor-tyrosine kinase fusion genes that cause chronic myeloid malignancies associated with hypereosinophilia. The aim of this work was to gain insight into the mechanisms whereby fusion genes affect human hematopoietic cells and in particular the eosinophil lineage.We introduced ETV6-PDGFRB and FIP1L1-PDGFRA into human CD34(+) hematopoietic progenitor and stem cells isolated from umbilical cord blood.Cells transduced with these oncoge  ...[more]

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