Transcriptomics

Dataset Information

30

Reprogramming is achieved within a single cell cycle after mouse nuclear transfer


ABSTRACT: Although nuclear transfer allows the reprogramming of somatic cells to totipotency, little is known concerning the kinetics by which it takes place or the minimum requirements for its success. Here, we demonstrate that reprogramming can be achieved within a few hours and a single cell-cycle as long as two key constraints on reprogramming are satisfied. First, the recipient cell chromosomes must be removed during mitosis. Second, the nuclear envelope of the donor cell must be broken down and its chromosomes condensed, allowing an embryonic nucleus to be constructed around the incoming chromosomes. If these requirements are not met, then reprogramming fails and embryonic development arrests. These results point to a central role for processes intimately linked to cell division in mediating efficient transitions between transcriptional programs. tail tip skin fibroblasts were transferred into mitotic mouse zygotes, blastomeres or oocytes.

ORGANISM(S): Mus musculus  

SUBMITTER: Dieter Egli  Kevin Eggan    

PROVIDER: E-GEOD-28811 | ArrayExpress| 2011-10-12

SECONDARY ACCESSION(S): GSE28811PRJNA138795

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2011-10-04 | GSE28811 | GEO
2014-05-01 | E-GEOD-21609 | ArrayExpress
2011-10-17 | E-GEOD-27507 | ArrayExpress
2011-10-12 | E-GEOD-24631 | ArrayExpress
2011-10-17 | E-GEOD-28022 | ArrayExpress
2012-07-17 | E-GEOD-20503 | ArrayExpress
2011-10-17 | E-GEOD-28024 | ArrayExpress
2012-10-22 | E-GEOD-41345 | ArrayExpress
2015-08-19 | E-GEOD-55939 | ArrayExpress
2014-05-01 | E-GEOD-20477 | ArrayExpress