Transcriptomics

Dataset Information

38

Transcriptomic and proteomic profiling of KEAP1 disrupted and sulforaphane treated human breast epithelial cells


ABSTRACT: Sulforaphane (SFN), an isothiocyanate found in cruciferous vegetables, is a potent inhibitor of experimental mammary carcinogenesis and may be an effective, safe chemopreventive agent for use in humans. SFN acts in part on the Keap1/Nrf2 pathway to regulate a battery of cytoprotective genes. In this study transcriptomic and proteomic changes in the estrogen receptor negative, non tumorigenic human breast epithelial MCF10A cell line were analyzed following SFN treatment or KEAP1 knockdown with siRNA using microarray and stable isotopic labeling with amino acids in culture (SILAC), respectively. Changes in selected transcripts and proteins were confirmed by PCR and Western blot in MCF10A and MCF12A cells. There was strong correlation between the transcriptomic and proteomic responses in both the SFN treatment (R=0.679, P<0.05) and KEAP1 knockdown (R=0.853, P<0.05) experiments. Common pathways for SFN treatment and KEAP1 knockdown were xenobiotic metabolism and antioxidants, glutathione metabolism, carbohydrate metabolism and NADH/NADPH regeneration. Moreover, these pathways were most prominent in both the transcriptomic and proteomic analyses. The aldo-keto reductase family members, AKR1B10, AKR1C1, AKR1C2 and AKR1C3, as well as NQO1 and ALDH3A1, were highly upregulated at both the transcriptomic and proteomic level. Collectively, these studies served to identify potential biomarkers that can be used in clinical trials to investigate the initial pharmacodynamic action of SFN in the breast. MCF10A cells were treated with SFN or had KEAP1 knocked down by siRNA.

ORGANISM(S): Homo sapiens  

SUBMITTER: Abena S Agyeman  Abena Agyeman    

PROVIDER: E-GEOD-28813 | ArrayExpress | 2011-05-18

SECONDARY ACCESSION(S): GSE28813PRJNA138799

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2011-04-22 | GSE28813 | GEO
2014-05-04 | E-GEOD-56186 | ArrayExpress
2015-03-16 | E-GEOD-59792 | ArrayExpress
2011-12-06 | E-TABM-477 | ArrayExpress
2016-07-20 | PXD001463 | Pride
2018-04-04 | PXD008343 | Pride
2016-08-25 | PXD000332 | Pride
2017-11-27 | E-MTAB-4893 | ArrayExpress
2017-01-02 | PXD004151 | Pride
2011-11-27 | E-GEOD-26264 | ArrayExpress