Dataset Information


MRNA-seq of Human Airway Epithelial Cells

ABSTRACT: mRNA expression was profiled from pooled bronchial airway epithelial cell brushings (n=3 patients/pool) obtained during bronchoscopy from healthy never (NS) and current smokers (S) and smokers with (C) and without (NC) lung cancer 4 samples were sequened, each representing a pool of 3 patients. The phenotypes of the 4 samples were as follows: healthy non-smoker, healthy smoker, smoker without lung cancer and smoker with lung cancer.

ORGANISM(S): Homo sapiens  

SUBMITTER: Jennifer Beane   Avrum Spira 

PROVIDER: E-GEOD-29006 | ArrayExpress | 2011-06-01



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Characterizing the impact of smoking and lung cancer on the airway transcriptome using RNA-Seq.

Beane Jennifer J   Vick Jessica J   Schembri Frank F   Anderlind Christina C   Gower Adam A   Campbell Joshua J   Luo Lingqi L   Zhang Xiao Hui XH   Xiao Ji J   Alekseyev Yuriy O YO   Wang Shenglong S   Levy Shawn S   Massion Pierre P PP   Lenburg Marc M   Spira Avrum A  

Cancer prevention research (Philadelphia, Pa.) 20110601 6

Cigarette smoke creates a molecular field of injury in epithelial cells that line the respiratory tract. We hypothesized that transcriptome sequencing (RNA-Seq) will enhance our understanding of the field of molecular injury in response to tobacco smoke exposure and lung cancer pathogenesis by identifying gene expression differences not interrogated or accurately measured by microarrays. We sequenced the high-molecular-weight fraction of total RNA (>200 nt) from pooled bronchial airway epithelia  ...[more]

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