Transcriptomics

Dataset Information

2

Technical and biological variance structure in mRNA-Seq data


ABSTRACT: mRNA expression data from next generation sequencing platforms is obtained in the form of counts per gene or exon. Counts are generally assumed to follow a Poisson distribution in which the variance is equal to the mean. However, it is common in observed count data with biological variation for over-dispersion to be present, i.e., for the variance to be greater than the mean. examination of technical and biological variance in mRNA-seq count data

ORGANISM(S): Homo sapiens  

SUBMITTER: Brian Bot   Ann Oberg  Gregory Poland 

PROVIDER: E-GEOD-29022 | ArrayExpress | 2012-07-16

SECONDARY ACCESSION(S): GSE29022PRJNA140475

REPOSITORIES: GEO, ArrayExpress

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Publications

Technical and biological variance structure in mRNA-Seq data: life in the real world.

Oberg Ann L AL   Bot Brian M BM   Grill Diane E DE   Poland Gregory A GA   Therneau Terry M TM  

BMC genomics 20120707


mRNA expression data from next generation sequencing platforms is obtained in the form of counts per gene or exon. Counts have classically been assumed to follow a Poisson distribution in which the variance is equal to the mean. The Negative Binomial distribution which allows for over-dispersion, i.e., for the variance to be greater than the mean, is commonly used to model count data as well.In mRNA-Seq data from 25 subjects, we found technical variation to generally follow a Poisson distributio  ...[more]

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