Dataset Information


Calcineurin/NFAT signaling inhibits myeloid hematopoiesis in mice.

ABSTRACT: Nuclear factor of activated T cells (NFAT) comprises a family of transcription factors that regulate T cell development, activation and differentiation. NFAT signaling can also mediate granulocyte and DC activation, but it is unknown whether NFAT influences their development from progenitors. Here we report a novel role for calcineurin/NFAT signaling as a negative regulator of myeloid hematopoiesis. Reconstituting lethally-irradiated mice with hematopoietic stem cells expressing an NFAT-inhibitory peptide resulted in enhanced development of the myeloid compartment. Culturing bone marrow cells with Flt3-L and Cyclosporin A, which inhibits NFAT signaling, increased numbers of differentiated DC. Global gene expression analysis of untreated DC and NFAT-inhibited DC revealed differential expression of transcripts that regulate cell cycle and apoptosis. Thus, calcineurin/NFAT signaling negatively regulates myeloid lineage development. The finding that NFAT acts as a negative regulator of myeloid development provides novel insight in understanding immune responses during treatment with calcineurin/NFAT inhibitors as Cyclosporin A. bone marrow cells from C57B/6 mice were stimulated in Flt3-L suplemented media in presence or absence of calcineurin/NFAT inhibitor Cyclosporin A, samples in 3 biological replicates

ORGANISM(S): Mus musculus  

SUBMITTER: Jan Fric  Benjamin Hofmann   Siti A Isa   jinmiao chen   Alessandra Mortellaro   Clarice Lim   Jinmiao Chen   Esther Koh   Paola Ricciardi-Castagnoli   Christiane Ruedl    

PROVIDER: E-GEOD-29094 | ArrayExpress | 2011-10-12



altmetric image


Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

| GSE47208 | GEO
2010-11-08 | GSE25175 | GEO
2014-05-02 | E-GEOD-25175 | ArrayExpress
2017-05-02 | E-MTAB-5258 | ArrayExpress
2015-12-26 | E-GEOD-58590 | ArrayExpress
| PRJNA140321 | ENA
| GSE71936 | GEO
2012-03-27 | E-GEOD-21499 | ArrayExpress
| GSE67739 | GEO
2010-07-15 | GSE21499 | GEO