Dataset Information


Identification of response genes upon neuronal activation in mouse cortical neurons

ABSTRACT: We search for developmental changes specific to humans by examining gene expression profiles in the human, chimpanzee and rhesus macaque prefrontal and cerebellar cortex. In both brain regions, developmental patterns were more evolved in humans than in chimpanzees. The major human specific genes in prefrontal cortex was enriched in neuronal functions and regulated by several transcription factors, which were previously implicated in regulation of neuronal functions. To confirm neuronal function of the human prefrontal cortex specific genes, we identifed response genes upon neuronal activation in mouse cortical neurons. Our results show that human specific genes are enriched in the response genes upon neuronal activation, implying the function of human prefrontal cortex specific genes in synaptic development. The cortical neurons from E15 mouse were isolated and cultured. We then exposed neurons to bicuculline (Bic), or potassium chloride (KCl), or without treatment. The cultured neurons under each group were hybridized to Agilent whole mouse genome oligo microarray (4x44k).

ORGANISM(S): Mus Musculus

SUBMITTER: Xiling Liu   Philipp Khaitovich  Mehmet Somel  Zilong Qiu 

PROVIDER: E-GEOD-29139 | ArrayExpress | 2012-02-02



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Extension of cortical synaptic development distinguishes humans from chimpanzees and macaques.

Liu Xiling X   Somel Mehmet M   Tang Lin L   Yan Zheng Z   Jiang Xi X   Guo Song S   Yuan Yuan Y   He Liu L   Oleksiak Anna A   Zhang Yan Y   Li Na N   Hu Yuhui Y   Chen Wei W   Qiu Zilong Z   Pääbo Svante S   Khaitovich Philipp P  

Genome research 20120202 4

Over the course of ontogenesis, the human brain and human cognitive abilities develop in parallel, resulting in a phenotype strikingly distinct from that of other primates. Here, we used microarrays and RNA-sequencing to examine human-specific gene expression changes taking place during postnatal brain development in the prefrontal cortex and cerebellum of humans, chimpanzees, and rhesus macaques. We show that the most prominent human-specific expression change affects genes associated with syna  ...[more]

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