Dataset Information


Folic acid supplementation dysregulates gene expression in lymphoblastoid cells

ABSTRACT: To prove our hypothesis that excess folic acid supplementation is responsible for widespread gene expression changes during gestational development that may be responsible for abnormal behaviors later on in the life, we have used whole genome microarray expression profiling in lymphoblstoid cells as a proof of concept. Human lymphoblastoid cells in culture were supplemented with folic acid, and four days later gene expression changes were measured. Expression of three genes (FMR1, GPR37L1 and TSSK3) from this data was confirmed by Western blot analyses. Dysregulation of other genes (ARHGAP6, EF1A1, MEST, SMAD3) was confirmed by qRT-PCR. Lymphoid cells at confluence were grown with or without folic acid for four days. Total mRNA was prepared and labeled with cyanine-3 (Cy3), followed by hybridization. RNA samples from three independent experiments were pooled.


ORGANISM(S): Homo sapiens  

SUBMITTER: W T Brown   Raju K Pullarkat  Mohammed A Junaid  Mohammed A. Junaid  Salomon Kuizon 

PROVIDER: E-GEOD-29141 | ArrayExpress | 2011-08-23



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Folic acid supplementation dysregulates gene expression in lymphoblastoid cells--implications in nutrition.

Junaid Mohammed A MA   Kuizon Salomon S   Cardona Juan J   Azher Tayaba T   Murakami Noriko N   Pullarkat Raju K RK   Brown W Ted WT  

Biochemical and biophysical research communications 20110816 4

For over a decade, folic acid (FA) supplementation has been widely prescribed to pregnant women to prevent neural tube closure defects in newborns. Although neural tube closure occurs within the first trimester, high doses of FA are given throughout pregnancy, the physiological consequences of which are unknown. FA can cause epigenetic modification of the cytosine residues in the CpG dinucleotide, thereby affecting gene expression. Dysregulation of crucial gene expression during gestational deve  ...[more]

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