Dataset Information


Bglu3 congenic mice - gene expression in liver

ABSTRACT: A congenic line was constructed by introgressing a C3H chromosome 1 region harboring Bglu3 into C57BL/6 apoE-/- background. RNA was extracted from liver using a QIAGEN kit . Total RNA was pooled in an equal amount from 3 mice for each group. Standard Affymetrix procedures were performed using 8ug of total RNA. Microarrays were used to detect gene expression in the liver of Bglu3 congenic mice and C57BL/6 apoE-deficient mouse strains fed a western diet. 2 groups of mice were studied: C57BL/6 apoE-/- control mice and Bglu3 congenic mice. After fed a Western diet for 12 weeks, liver was harvested and total RNA prepared.

ORGANISM(S): Mus musculus  

SUBMITTER: Jing Li   qian wang  Qian Wang  Weibin Shi 

PROVIDER: E-GEOD-29151 | ArrayExpress | 2011-05-11



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Characterization of Bglu3, a mouse fasting glucose locus, and identification of Apcs as an underlying candidate gene.

Li Jing J   Lu Zongji Z   Wang Qian Q   Su Zhiguang Z   Bao Yongde Y   Shi Weibin W  

Physiological genomics 20120124 6

Bglu3 is a quantitative trait locus for fasting glucose on distal chromosome 1 identified in an intercross between C57BL/6 (B6) and C3H/HeJ (C3H) apolipoprotein E-deficient (apoE(-/-)) mice. This locus was subsequently replicated in two separate mouse intercrosses. The objective of this study was to characterize Bglu3 through construction and analysis of a congenic strain and identify underlying candidate genes. Congenic mice were constructed by introgressing a genomic region harboring Bglu3 fro  ...[more]

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