Genomics

Dataset Information

296

Genome-wide location analysis of WNT (Tcf7l2) and BMP (SMAD1) in human hematopoeitic progenitors co-occupied with lineage specific regulators (GATA1, GATA2)


ABSTRACT: Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration. This represents the human CD34 ChIP-seq portion of this dataset. Human hematopoietic cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total Smad1 (Santa Cruz SC-7965), Tcf7l2 (Santa Cruz SC-8631),Gata1 (Santa Cruz SC-265), Gata2 (Santa Cruz SC-9008) or CEBPA (SC-9314).

ORGANISM(S): Homo sapiens  

SUBMITTER: T V Bowman   E Trompouki  J L Leblanc  C S Martin  R F Paulson  A DiBiase  C Mosimann  D Wu  L I Zon  Teresa Venezia Bowman  P Li  L N Lawton  E M Durand  A K Sessa  R A Young  J N Cech  G C Heffner  G Q Daley  Z P Fan 

PROVIDER: E-GEOD-29194 | ArrayExpress | 2011-10-07

SECONDARY ACCESSION(S): SRP008214GSE29194PRJNA142953

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications


BMP and Wnt signaling pathways control essential cellular responses through activation of the transcription factors SMAD (BMP) and TCF (Wnt). Here, we show that regeneration of hematopoietic lineages following acute injury depends on the activation of each of these signaling pathways to induce expression of key blood genes. Both SMAD1 and TCF7L2 co-occupy sites with master regulators adjacent to hematopoietic genes. In addition, both SMAD1 and TCF7L2 follow the binding of the predominant lineage  ...[more]

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